Protein profile

KP13_03673

DNA-binding protein HU-beta

Genome: KpKP13

Gene: hupB AHE46013.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GP22
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Amino acids 90
Annotations 9
Features 18
PDB binders 0
Druggability 0.404

Overview

Basic information about this protein and its source genome.

Accession
KP13_03673
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
hupB AHE46013.1
Status
annotated
Amino acids
90
Structure source
AlphaFold + ColabFold
GO
GO:0030527 The action of a molecule that contributes to the structural integrity of chromatin. GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:1990103 A protein-DNA complex containing DNA-bound DnaA attached to HU. HU is a dimer encoded by two closely related genes. Essential for the initiation of replication in bacteria; stimulates the DnaA-dependent unwinding of oriC. GO:1990178 A protein-DNA complex that consists of HU heterodimers (an alpha and a beta chain) assembled into octamers along DNA. HU binds to double-stranded DNA in a structure- and sequence-specific manner and bends the DNA into a nucleosome-like structure. GO:0042802 Binding to an identical protein or proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
71.111
DEG E-value
5.97e-39
Localization
Cytoplasmic
ColabFold pLDDT
96.7

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.404
Structure A0A0H3GP22
Pocket Pocket 1
P2Rank
Structure A0A0H3GP22
Pocket No pockets
ColabFold model
FPocket 0.432 · Pocket 3
P2Rank 0.008 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 1836 / 4744 genomes with a hit
Normalized 0.387

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

9 GO

Gene Ontology (GO)

9
  • GO:0030527 The action of a molecule that contributes to the structural integrity of chromatin.
  • GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:1990103 A protein-DNA complex containing DNA-bound DnaA attached to HU. HU is a dimer encoded by two closely related genes. Essential for the initiation of replication in bacteria; stimulates the DnaA-dependent unwinding of oriC.
  • GO:1990178 A protein-DNA complex that consists of HU heterodimers (an alpha and a beta chain) assembled into octamers along DNA. HU binds to double-stranded DNA in a structure- and sequence-specific manner and bends the DNA into a nucleosome-like structure.
  • GO:0042802 Binding to an identical protein or proteins.
  • GO:0030261 The progressive compaction of dispersed interphase chromatin into threadlike chromosomes prior to mitotic or meiotic nuclear division, or during apoptosis, in eukaryotic cells.
  • GO:0006270 The process in which DNA-dependent DNA replication is started; it begins when specific sequences, known as origins of replication, are recognized and bound by the origin recognition complex, followed by DNA unwinding.
  • GO:0006351 The synthesis of an RNA transcript from a DNA template.

Sequence Features

Domain/signature hits from InterPro and related databases.

18 records
Show feature table
Start End DB Term Name
1 90 Gene3D G3DSA:4.10.520.10 -
1 90 InterPro IPR010992 Integration host factor (IHF)-like DNA-binding domain superfamily
1 90 SUPERFAMILY SSF47729 IHF-like DNA-binding proteins
46 65 ProSitePatterns PS00045 Bacterial histone-like DNA-binding proteins signature.
46 65 InterPro IPR020816 Histone-like DNA-binding protein, conserved site
1 89 PANTHER PTHR33175 DNA-BINDING PROTEIN HU
1 89 InterPro IPR000119 Histone-like DNA-binding protein
2 87 CDD cd13831 HU
1 90 Pfam PF00216 Bacterial DNA-binding protein
1 90 InterPro IPR000119 Histone-like DNA-binding protein
1 90 FunFam G3DSA:4.10.520.10:FF:000001 DNA-binding protein HU
74 88 PRINTS PR01727 Prokaryotic integration host factor signature
74 88 InterPro IPR000119 Histone-like DNA-binding protein
40 55 PRINTS PR01727 Prokaryotic integration host factor signature
40 55 InterPro IPR000119 Histone-like DNA-binding protein
58 71 PRINTS PR01727 Prokaryotic integration host factor signature
58 71 InterPro IPR000119 Histone-like DNA-binding protein
1 90 SMART SM00411 bhlneu

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GP22
AlphaFold — — full sequence — Viewing
ColabFold KP13_03673
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.404