Protein profile

KP13_03687

Protoheme IX farnesyltransferase

Genome: KpKP13

Gene: cyoE AHE46025.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GKG4
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Amino acids 295
Annotations 7
Features 42
PDB binders 0
Druggability 0.857

Overview

Basic information about this protein and its source genome.

Accession
KP13_03687
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
cyoE AHE46025.1
Status
annotated
Amino acids
295
Structure source
AlphaFold + ColabFold
EC
2.5.1.141
GO
GO:0016765 Catalysis of the transfer of an alkyl or aryl (but not methyl) group from one compound (donor) to another (acceptor). GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0008495 Catalysis of the reaction: protoheme IX + (2E,6E)-farnesyl diphosphate + H2O = heme O + diphosphate. GO:0006783 The chemical reactions and pathways resulting in the formation of heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring, from less complex precursors. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0048034 The chemical reactions and pathways resulting in the formation of heme O, a derivative of heme containing a 17-carbon hydroxyethylfarnesyl side chain at position 8 of the tetrapyrrole macrocycle.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
37.5
Human E-value
3.73e-27
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
95.07

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.857
Structure A0A0H3GKG4
Pocket Pocket 1
P2Rank 0.984
Structure A0A0H3GKG4
Pocket Pocket 1
ColabFold model
FPocket 0.762 · Pocket 1
P2Rank 0.985 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 125 / 4744 genomes with a hit
Normalized 0.026

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0016765 Catalysis of the transfer of an alkyl or aryl (but not methyl) group from one compound (donor) to another (acceptor).
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008495 Catalysis of the reaction: protoheme IX + (2E,6E)-farnesyl diphosphate + H2O = heme O + diphosphate.
  • GO:0006783 The chemical reactions and pathways resulting in the formation of heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring, from less complex precursors.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0048034 The chemical reactions and pathways resulting in the formation of heme O, a derivative of heme containing a 17-carbon hydroxyethylfarnesyl side chain at position 8 of the tetrapyrrole macrocycle.

Sequence Features

Domain/signature hits from InterPro and related databases.

42 records
Show feature table
Start End DB Term Name
5 27 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
32 36 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
37 59 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
234 251 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
3 281 NCBIfam TIGR01473 heme o synthase
3 281 InterPro IPR006369 Protohaem IX farnesyltransferase
133 155 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 283 Hamap MF_00154 Protoheme IX farnesyltransferase [cyoE].
1 283 InterPro IPR006369 Protohaem IX farnesyltransferase
127 132 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
107 126 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
3 281 PANTHER PTHR43448 PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL
6 277 CDD cd13957 PT_UbiA_Cox10
6 277 InterPro IPR006369 Protohaem IX farnesyltransferase
12 31 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
37 59 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
80 101 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
263 285 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
133 155 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
156 160 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
232 251 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
107 126 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
18 267 Pfam PF01040 UbiA prenyltransferase family
18 267 InterPro IPR000537 UbiA prenyltransferase family
60 79 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
252 262 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
208 226 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
183 207 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
13 181 Gene3D G3DSA:1.10.357.140 UbiA prenyltransferase
13 181 InterPro IPR044878 UbiA prenyltransferase superfamily
161 182 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
160 182 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
56 78 ProSitePatterns PS00943 UbiA prenyltransferase family signature.
56 78 InterPro IPR030470 UbiA prenyltransferase conserved site
227 231 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
208 230 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
285 295 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
102 106 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
263 284 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
9 163 FunFam G3DSA:1.10.357.140:FF:000001 Protoheme IX farnesyltransferase
80 102 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GKG4
AlphaFold — — full sequence — Viewing
ColabFold KP13_03687
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.857

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 40.73 0.964
2 3.83 0.149
3 3.36 0.119
4 1.78 0.033
5 1.59 0.025