Protein profile

KP13_02921

Na(+)-translocating NADH-quinone reductase subunit E

Genome: KpKP13

Gene: nqrE AHE46197.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GNQ5
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Amino acids 198
Annotations 8
Features 28
PDB binders 0
Druggability 0.927

Overview

Basic information about this protein and its source genome.

Accession
KP13_02921
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
nqrE AHE46197.1
Status
annotated
Amino acids
198
Structure source
AlphaFold + ColabFold
EC
7.2.1.1
GO
GO:0016655 Catalysis of an oxidation-reduction (redox) reaction in which NADH or NADPH acts as a hydrogen or electron donor and reduces a quinone or a similar acceptor molecule. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0022904 A process in which a series of electron carriers operate together to transfer electrons from donors such as NADH and FADH2 to any of several different terminal electron acceptors to generate a transmembrane electrochemical gradient. GO:0009276 The peptidoglycan layer of the Gram-negative cell envelope. In Gram-negative cells the peptidoglycan is relatively thin (1-2nm) and is linked to the outer membrane by lipoproteins. In Gram-negative cells the peptidoglycan is too thin to retain the primary stain in the Gram staining procedure and therefore cells appear red after Gram stain. GO:0012505 A collection of membranous structures involved in transport within the cell. The main components of the endomembrane system are endoplasmic reticulum, Golgi bodies, vesicles, cell membrane and nuclear envelope. Members of the endomembrane system pass materials through each other or though the use of vesicles. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
45.361
DEG E-value
5.299999999999999e-53
Localization
CytoplasmicMembrane
ColabFold pLDDT
87.14

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.927
Structure A0A0H3GNQ5
Pocket Pocket 3
P2Rank 0.006
Structure A0A0H3GNQ5
Pocket Pocket 1
ColabFold model
FPocket 0.87 · Pocket 6
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 667 / 4744 genomes with a hit
Normalized 0.141

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0016655 Catalysis of an oxidation-reduction (redox) reaction in which NADH or NADPH acts as a hydrogen or electron donor and reduces a quinone or a similar acceptor molecule.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0022904 A process in which a series of electron carriers operate together to transfer electrons from donors such as NADH and FADH2 to any of several different terminal electron acceptors to generate a transmembrane electrochemical gradient.
  • GO:0009276 The peptidoglycan layer of the Gram-negative cell envelope. In Gram-negative cells the peptidoglycan is relatively thin (1-2nm) and is linked to the outer membrane by lipoproteins. In Gram-negative cells the peptidoglycan is too thin to retain the primary stain in the Gram staining procedure and therefore cells appear red after Gram stain.
  • GO:0012505 A collection of membranous structures involved in transport within the cell. The main components of the endomembrane system are endoplasmic reticulum, Golgi bodies, vesicles, cell membrane and nuclear envelope. Members of the endomembrane system pass materials through each other or though the use of vesicles.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0006814 The directed movement of sodium ions (Na+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

Sequence Features

Domain/signature hits from InterPro and related databases.

28 records
Show feature table
Start End DB Term Name
4 196 Pfam PF02508 Rnf-Nqr subunit, membrane protein
4 196 InterPro IPR003667 NqrDE/RnfAE
1 11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
75 94 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
161 171 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
32 37 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
41 63 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 197 PANTHER PTHR30335 INTEGRAL MEMBRANE PROTEIN OF SOXR-REDUCING COMPLEX
3 197 NCBIfam TIGR01940 NADH:ubiquinone reductase (Na(+)-transporting) subunit E
3 197 InterPro IPR010967 Na(+)-translocating NADH-quinone reductase subunit E
140 160 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
56 74 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
95 105 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
38 55 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
194 198 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 198 Hamap MF_00429 Na(+)-translocating NADH-quinone reductase subunit E [nqrE].
1 198 InterPro IPR010967 Na(+)-translocating NADH-quinone reductase subunit E
1 198 PIRSF PIRSF006102 NQR_DE
1 198 InterPro IPR003667 NqrDE/RnfAE
106 128 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
172 193 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
12 31 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
139 161 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
129 139 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
171 193 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
12 31 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
76 98 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
108 130 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GNQ5
AlphaFold — — full sequence — Viewing
ColabFold KP13_02921
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.927

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 1.94 0.04
2 1.02 0.006
3 0.59 0.0