Protein profile

KP13_01820

Vitamin B12-binding protein

Genome: KpKP13

Gene: AHE46260.1 btuF Structure source: AlphaFold + ColabFold UniProt A0A0H3GII9
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Amino acids 266
Annotations 3
Features 21
PDB binders 0
Druggability 0.384

Overview

Basic information about this protein and its source genome.

Accession
KP13_01820
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE46260.1 btuF
Status
annotated
Amino acids
266
Structure source
AlphaFold + ColabFold
GO
GO:0042597 The region between the inner (cytoplasmic) and outer membrane (Gram-negative Bacteria) or cytoplasmic membrane and cell wall (Fungi and Gram-positive Bacteria). GO:0015889 The directed movement of cobalamin (vitamin B12), a water-soluble vitamin characterized by possession of a corrin nucleus containing a cobalt atom, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0031419 Binding to cobalamin (vitamin B12), a water-soluble vitamin characterized by possession of a corrin nucleus containing a cobalt atom.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Unknown
ColabFold pLDDT
92.67

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.384
Structure A0A0H3GII9
Pocket Pocket 11
P2Rank 0.281
Structure A0A0H3GII9
Pocket Pocket 1
ColabFold model
FPocket 0.435 · Pocket 5
P2Rank 0.387 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 107 / 4744 genomes with a hit
Normalized 0.023

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Gene Ontology (GO)

3
  • GO:0042597 The region between the inner (cytoplasmic) and outer membrane (Gram-negative Bacteria) or cytoplasmic membrane and cell wall (Fungi and Gram-positive Bacteria).
  • GO:0015889 The directed movement of cobalamin (vitamin B12), a water-soluble vitamin characterized by possession of a corrin nucleus containing a cobalt atom, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0031419 Binding to cobalamin (vitamin B12), a water-soluble vitamin characterized by possession of a corrin nucleus containing a cobalt atom.

Sequence Features

Domain/signature hits from InterPro and related databases.

21 records
Show feature table
Start End DB Term Name
1 39 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
2 266 Hamap MF_01000 Vitamin B12-binding protein [btuF].
2 266 InterPro IPR023544 ABC transporter, vitamin B12-binding protein
25 260 CDD cd01144 BtuF
1 22 SignalP_EUK SignalP-noTM SignalP-noTM
25 262 SUPERFAMILY SSF53807 Helical backbone metal receptor
1 3 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
21 139 Gene3D G3DSA:3.40.50.1980 Nitrogenase molybdenum iron protein domain
16 22 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
1 22 SignalP_GRAM_NEGATIVE SignalP-TM SignalP-TM
26 225 Pfam PF01497 Periplasmic binding protein
26 225 InterPro IPR002491 ABC transporter periplasmic binding domain
25 266 ProSiteProfiles PS50983 Iron siderophore/cobalamin periplasmic-binding domain profile.
25 266 InterPro IPR002491 ABC transporter periplasmic binding domain
7 29 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
141 266 Gene3D G3DSA:3.40.50.1980 Nitrogenase molybdenum iron protein domain
23 266 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
17 264 PANTHER PTHR42860 VITAMIN B12-BINDING PROTEIN
4 15 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
1 22 Phobius SIGNAL_PEPTIDE Signal peptide region
25 217 NCBIfam NF038402 helical backbone metal receptor

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GII9
AlphaFold — — full sequence — Viewing
ColabFold KP13_01820
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
11 0.384

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 3.58 0.133
2 2.98 0.097