Overview
Basic information about this protein and its source genome.
- Accession
- KP13_01834
- Gene
- pulA AHE46274.1
- Status
- annotated
- Amino acids
- 1037
- Structure source
- AlphaFold + ColabFold
Target profile
Computed evidence for target prioritization.
- Human off-target
- No hit
- Human identity (%)
- 0.0
- Gut microbiome off-target
- hit
- Essential (DEG)
- N
- DEG identity (%)
- 0.0
- Localization
- Unknown
- ColabFold pLDDT
- 97.88
Selected Druggability evidence
AlphaFold / UniProt modelSelected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.
Sequence
Primary amino-acid sequence viewer.
Functional Annotations
Enzyme classification and Gene Ontology terms linked to this protein.
Enzyme Commission (EC)
1Gene Ontology (GO)
4- GO:0030246 Binding to a carbohydrate, which includes monosaccharides, oligosaccharides and polysaccharides as well as substances derived from monosaccharides by reduction of the carbonyl group (alditols), by oxidation of one or more hydroxy groups to afford the corresponding aldehydes, ketones, or carboxylic acids, or by replacement of one or more hydroxy group(s) by a hydrogen atom. Cyclitols are generally not regarded as carbohydrates.
- GO:0005975 The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y.
- GO:0004553 Catalysis of the hydrolysis of any O-glycosyl bond.
- GO:0051060 Catalysis of the hydrolysis of (1,6)-alpha-D-glucosidic linkages in pullulan (a linear polymer of alpha-(1,6)-linked maltotriose units) and in amylopectin and glycogen, and the a- and b-limit dextrins of amylopectin and glycogen.
Sequence Features
Domain/signature hits from InterPro and related databases.
Show feature table
| Start | End | DB | Term | Name |
|---|---|---|---|---|
| 127 | 241 | Gene3D | G3DSA:2.60.40.1130 | - |
| 251 | 353 | SUPERFAMILY | SSF81296 | E set domains |
| 251 | 353 | InterPro | IPR014756 | Immunoglobulin E-set |
| 6 | 118 | CDD | cd10315 | CBM41_pullulanase |
| 6 | 118 | InterPro | IPR005323 | Pullulanase, carbohydrate-binding module 41 |
| 347 | 919 | SUPERFAMILY | SSF51445 | (Trans)glycosidases |
| 347 | 919 | InterPro | IPR017853 | Glycoside hydrolase superfamily |
| 184 | 977 | PANTHER | PTHR43002 | GLYCOGEN DEBRANCHING ENZYME |
| 1 | 117 | Gene3D | G3DSA:2.60.40.1110 | - |
| 929 | 1037 | Gene3D | G3DSA:2.60.40.1180 | - |
| 929 | 1037 | InterPro | IPR013780 | Glycosyl hydrolase, all-beta |
| 128 | 1024 | NCBIfam | TIGR02103 | pullulanase-type alpha-1,6-glucosidase |
| 128 | 1024 | InterPro | IPR011839 | Alpha-1,6-glucosidases, pullulanase-type |
| 860 | 1020 | Pfam | PF11852 | Alpha-1,6-glucosidases, pullulanase-type, C-terminal |
| 860 | 1020 | InterPro | IPR024561 | Alpha-1,6-glucosidases, pullulanase-type, C-terminal |
| 252 | 350 | CDD | cd02860 | E_set_Pullulanase |
| 480 | 799 | SMART | SM00642 | aamy |
| 480 | 799 | InterPro | IPR006047 | Glycosyl hydrolase, family 13, catalytic domain |
| 436 | 509 | Pfam | PF18494 | Pullulanase Ins domain |
| 436 | 509 | InterPro | IPR041111 | Pullulanase, Ins domain |
| 120 | 240 | SUPERFAMILY | SSF81296 | E set domains |
| 120 | 240 | InterPro | IPR014756 | Immunoglobulin E-set |
| 6 | 115 | SUPERFAMILY | SSF49452 | Starch-binding domain-like |
| 6 | 115 | InterPro | IPR013784 | Carbohydrate-binding-like fold |
| 350 | 928 | Gene3D | G3DSA:3.20.20.80 | Glycosidases |
| 921 | 1036 | SUPERFAMILY | SSF51011 | Glycosyl hydrolase domain |
| 24 | 115 | Pfam | PF03714 | Bacterial pullanase-associated domain |
| 24 | 115 | InterPro | IPR005323 | Pullulanase, carbohydrate-binding module 41 |
| 131 | 240 | Pfam | PF17967 | Pullulanase N2 domain |
| 131 | 240 | InterPro | IPR040671 | Pullulanase, N2 domain |
| 378 | 866 | CDD | cd11341 | AmyAc_Pullulanase_LD-like |
| 251 | 335 | Pfam | PF02922 | Carbohydrate-binding module 48 (Isoamylase N-terminal domain) |
| 251 | 335 | InterPro | IPR004193 | Glycoside hydrolase, family 13, N-terminal |
| 242 | 349 | Gene3D | G3DSA:2.60.40.10 | Immunoglobulins |
| 242 | 349 | InterPro | IPR013783 | Immunoglobulin-like fold |
3D Structure
Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.
Loading 3D structure...
Structural evidence
0 + 2Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.
| Entry | Method | Resolution | Chain | Coverage | Links | Status |
|---|---|---|---|---|---|---|
|
AlphaFold
AF_A0A0H3GJK2
|
AlphaFold | — | — | full sequence | — | Viewing |
|
ColabFold
KP13_01834
|
ColabFold | — | — | full sequence | — | Loaded |
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer
Pockets (FPOCKET)
Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).
| FPOCKET | Sticks | Spheres | Surfaces | Druggability | Labels | Zoom | Positions |
|---|---|---|---|---|---|---|---|
| 10 | 0.302 |
Pockets (P2RANK)
Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).
| P2RANK | Sticks | Spheres | Surfaces | Score | Probability | Labels | Zoom | Positions |
|---|---|---|---|---|---|---|---|---|
| 1 | 14.87 | 0.737 | ||||||
| 2 | 7.9 | 0.419 | ||||||
| 3 | 3.78 | 0.146 | ||||||
| 4 | 3.1 | 0.104 | ||||||
| 5 | 2.71 | 0.081 |
Pockets (FPOCKET)
Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).
| FPOCKET | Sticks | Spheres | Surfaces | Druggability | Labels | Zoom | Positions |
|---|---|---|---|---|---|---|---|
| 21 | 0.405 |
Pockets (P2RANK)
Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).
| P2RANK | Sticks | Spheres | Surfaces | Score | Probability | Labels | Zoom | Positions |
|---|---|---|---|---|---|---|---|---|
| 1 | 20.85 | 0.863 | ||||||
| 2 | 8.01 | 0.426 | ||||||
| 3 | 4.23 | 0.173 | ||||||
| 4 | 4.07 | 0.163 | ||||||
| 5 | 3.09 | 0.103 |
Ligand evidence
Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.
Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.
No PDB structure with a co-crystallized ligand found for this exact protein.
Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.
| Ligand | Source crystal | UniProt (homolog) | MW · LogP · TPSA | Lipinski | PAINS | SMILES |
|---|---|---|---|---|---|---|
| TAM | A0A0C5GWS2 | 163.2 Da LogP -1.17 TPSA 86.7 | ✓ Ro5 | ✓ Clean |
C(CO)C(CCO)(CCO)N
|
Experimental bioactivity from ChEMBL measured directly on this protein. Score = pchembl (−log Ki/IC₅₀; higher = more potent).
No ChEMBL bioactivity data found for this exact protein.
Bioactivity inferred from similar proteins in ChEMBL. Score = pchembl (−log Ki/IC₅₀; higher = more potent).
No ChEMBL hits found through similar proteins.
Proposed virtual-screening candidates from ZINC. Score = Tanimoto similarity to a known binder (0–1; higher = more similar).
| Ligand | Tanimoto | MW · LogP · TPSA | Lipinski | PAINS | SMILES |
|---|---|---|---|---|---|
| ZINC2575038 | 0.625 | 205.3 Da LogP 0.00 TPSA 86.7 | ✓ Ro5 | ✓ Clean |
NC(CCCO)(CCCO)CCCO
|
PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.