Protein profile

KP13_01839

General secretion pathway protein F

Genome: KpKP13

Gene: pulF AHE46279.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GJJ6
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Amino acids 404
Annotations 6
Features 35
PDB binders 1
Druggability 0.906

Overview

Basic information about this protein and its source genome.

Accession
KP13_01839
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
pulF AHE46279.1
Status
annotated
Amino acids
404
Structure source
AlphaFold + ColabFold
GO
GO:0009306 The controlled release of proteins from a cell. GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0046872 Binding to a metal ion.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
52.84
DEG E-value
1.04e-151
Localization
CytoplasmicMembrane
ColabFold pLDDT
86.26

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.906
Structure A0A0H3GJJ6
Pocket Pocket 21
P2Rank 0.091
Structure A0A0H3GJJ6
Pocket Pocket 1
ColabFold model
FPocket 0.408 · Pocket 5
P2Rank 0.135 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 30 / 4744 genomes with a hit
Normalized 0.006

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0009306 The controlled release of proteins from a cell.
  • GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0046872 Binding to a metal ion.

Sequence Features

Domain/signature hits from InterPro and related databases.

35 records
Show feature table
Start End DB Term Name
222 241 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
255 368 Gene3D G3DSA:1.20.81.30 Type II secretion system (T2SS), domain F
255 368 InterPro IPR042094 Type II secretion system GspF domain superfamily
144 164 Coils Coil Coil
383 402 PRINTS PR00812 Bacterial general secretion pathway protein F signature
383 402 InterPro IPR003004 GspF/PilC family
65 77 PRINTS PR00812 Bacterial general secretion pathway protein F signature
65 77 InterPro IPR003004 GspF/PilC family
164 192 PRINTS PR00812 Bacterial general secretion pathway protein F signature
164 192 InterPro IPR003004 GspF/PilC family
367 381 PRINTS PR00812 Bacterial general secretion pathway protein F signature
367 381 InterPro IPR003004 GspF/PilC family
4 401 NCBIfam TIGR02120 type II secretion system inner membrane protein GspF
4 401 InterPro IPR011850 Type II secretion system protein GspF
164 192 ProSitePatterns PS00874 Bacterial type II secretion system protein F signature.
164 192 InterPro IPR001992 T2SS_GspF/T4SS_PilC conserved site
242 374 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
222 241 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
55 171 Gene3D G3DSA:1.20.81.30 Type II secretion system (T2SS), domain F
55 171 InterPro IPR042094 Type II secretion system GspF domain superfamily
168 191 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
168 190 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
255 371 FunFam G3DSA:1.20.81.30:FF:000001 Type II secretion system protein F
70 192 Pfam PF00482 Type II secretion system (T2SS), protein F
70 192 InterPro IPR018076 Type II secretion system protein GspF domain
273 393 Pfam PF00482 Type II secretion system (T2SS), protein F
273 393 InterPro IPR018076 Type II secretion system protein GspF domain
397 404 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
375 396 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
192 221 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 167 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
374 396 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 402 PANTHER PTHR30012 GENERAL SECRETION PATHWAY PROTEIN
1 402 InterPro IPR003004 GspF/PilC family
55 171 FunFam G3DSA:1.20.81.30:FF:000001 Type II secretion system protein F

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GJJ6
AlphaFold — — full sequence — Viewing
ColabFold KP13_01839
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
21 0.906
30 0.372
29 0.297

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 2.09 0.047
2 2.08 0.047
3 0.71 0.001

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

51 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
PE5
3C1Q
P45780 398.5 Da LogP 0.13 TPSA 94.1 ✓ Ro5 ✓ Clean CCOCCOCCOCCOCCOCCOCCOCCOCCO

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.