Protein profile

KP13_01842

General secretion pathway protein I

Genome: KpKP13

Gene: pulI AHE46282.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GIF6
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Amino acids 121
Annotations 3
Features 19
PDB binders 0
Druggability 0.611

Overview

Basic information about this protein and its source genome.

Accession
KP13_01842
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
pulI AHE46282.1
Status
annotated
Amino acids
121
Structure source
AlphaFold + ColabFold
GO
GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex. GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
93.29

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.611
Structure A0A0H3GIF6
Pocket Pocket 1
P2Rank 0.008
Structure A0A0H3GIF6
Pocket Pocket 1
ColabFold model
FPocket 0.328 · Pocket 6
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 7 / 4744 genomes with a hit
Normalized 0.001

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Gene Ontology (GO)

3
  • GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex.
  • GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Sequence Features

Domain/signature hits from InterPro and related databases.

19 records
Show feature table
Start End DB Term Name
1 5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
5 27 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
6 105 NCBIfam TIGR01707 type II secretion system minor pseudopilin GspI
6 105 InterPro IPR010052 Type II secretion system protein GspI
5 25 ProSitePatterns PS00409 Prokaryotic N-terminal methylation site.
5 25 InterPro IPR012902 Prokaryotic N-terminal methylation site
31 120 Gene3D G3DSA:3.30.1300.30 -
40 118 Pfam PF02501 Type II secretion system (T2SS), protein I
40 118 InterPro IPR003413 Type II secretion system protein GspI, C-terminal
28 121 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 117 PANTHER PTHR38779 TYPE II SECRETION SYSTEM PROTEIN I-RELATED
1 117 InterPro IPR010052 Type II secretion system protein GspI
1 27 Pfam PF07963 Prokaryotic N-terminal methylation motif
1 27 InterPro IPR012902 Prokaryotic N-terminal methylation site
37 118 SUPERFAMILY SSF54523 Pili subunits
37 118 InterPro IPR045584 Pilin-like
6 27 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
5 26 NCBIfam TIGR02532 prepilin-type N-terminal cleavage/methylation domain
5 26 InterPro IPR012902 Prokaryotic N-terminal methylation site

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GIF6
AlphaFold — — full sequence — Viewing
ColabFold KP13_01842
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.611
6 0.076
8 0.001
5 0.0

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 1.16 0.008