Protein profile

KP13_01845

General secretion pathway protein L

Genome: KpKP13

Gene: AHE46285.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GNF4
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Amino acids 397
Annotations 4
Features 15
PDB binders 0
Druggability 0.733

Overview

Basic information about this protein and its source genome.

Accession
KP13_01845
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE46285.1
Status
annotated
Amino acids
397
Structure source
AlphaFold + ColabFold
GO
GO:0009276 The peptidoglycan layer of the Gram-negative cell envelope. In Gram-negative cells the peptidoglycan is relatively thin (1-2nm) and is linked to the outer membrane by lipoproteins. In Gram-negative cells the peptidoglycan is too thin to retain the primary stain in the Gram staining procedure and therefore cells appear red after Gram stain. GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex. GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Unknown
ColabFold pLDDT
82.78

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.733
Structure A0A0H3GNF4
Pocket Pocket 11
P2Rank 0.049
Structure A0A0H3GNF4
Pocket Pocket 1
ColabFold model
FPocket 0.243 · Pocket 3
P2Rank 0.086 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 7 / 4744 genomes with a hit
Normalized 0.001

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Gene Ontology (GO)

4
  • GO:0009276 The peptidoglycan layer of the Gram-negative cell envelope. In Gram-negative cells the peptidoglycan is relatively thin (1-2nm) and is linked to the outer membrane by lipoproteins. In Gram-negative cells the peptidoglycan is too thin to retain the primary stain in the Gram staining procedure and therefore cells appear red after Gram stain.
  • GO:0015627 A large protein complex, containing 12-15 subunits, that spans the cell envelope of Gram-negative bacteria and mediates the movement of proteins into the extracellular environment. The complex includes a component in the cytoplasm, an inner membrane subcomplex that reaches into the periplasmic compartment and a secretion pore in the outer membrane. Proteins using the Type II pathway are transported across the cytoplasmic membrane by the Sec or Tat complex.
  • GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Sequence Features

Domain/signature hits from InterPro and related databases.

15 records
Show feature table
Start End DB Term Name
11 149 SUPERFAMILY SSF53067 Actin-like ATPase domain
11 149 InterPro IPR043129 ATPase, nucleotide binding domain
153 234 SUPERFAMILY SSF53067 Actin-like ATPase domain
153 234 InterPro IPR043129 ATPase, nucleotide binding domain
147 212 Gene3D G3DSA:3.30.420.370 -
12 236 Pfam PF05134 Type II secretion system (T2SS), protein L
12 236 InterPro IPR024230 GspL, cytoplasmic actin-ATPase-like domain
12 234 Gene3D G3DSA:3.30.420.380 -
6 396 PIRSF PIRSF015761 Protein_L
6 396 InterPro IPR007812 Type II secretion system protein GspL
242 394 Pfam PF12693 GspL periplasmic domain
242 394 InterPro IPR025691 GspL periplasmic domain
306 397 Gene3D G3DSA:3.30.1360.100 General secretion pathway protein M, EpsM
11 383 NCBIfam TIGR01709 type II secretion system protein GspL
11 383 InterPro IPR007812 Type II secretion system protein GspL

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GNF4
AlphaFold — — full sequence — Viewing
ColabFold KP13_01845
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
11 0.733
28 0.003
9 0.001
24 0.001

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 2.34 0.049
2 1.54 0.019
3 1.24 0.01