KpKP13 Protein target profile

Putative proline/betaine transporter

Accession: KP13_01985

Gene: proP AHE46423.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GIY8
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Length 434
Pocket druggability (P2Rank · AlphaFold DB model) 0.91
Functional annotation 0 EC 4 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Independent pocket support: P2Rank + FPocket Both predictors report a high-scoring binding site (P2Rank 0.91, FPocket 0.89). Use the 3D pocket comparison to verify whether they identify the same site. No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Strong predicted binding pocket FPocket selected-pocket score 0.89. This is a computational pocket signal, not binding validation. Review the complete evidence below

Risks to review

Microbiome similarity 128 of 4744 screened genomes (2.7%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.91) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
2.7% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
88.28 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.91
Structure A0A0H3GIY8
Pocket Pocket 1
Druggability (FPocket) 0.888
Structure A0A0H3GIY8
Pocket Pocket 5
ColabFold model
P2Rank 0.957 · Pocket 1
FPocket 0.909 · Pocket 9
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 128 / 4744 genomes with a hit
Prevalence 2.7%

Sequence

Primary amino-acid sequence viewer.

MSYANRPLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVVGELFFPADIPEWLRQVQTFGIFAAGYLARPLGGIIMAHFGDLVGRKKMFTLSILLMALPTLAIGLLPTYSSVGIIAPLLLLLMRILQGAAIGGEVPGAWVFVAEHVPEKRIGIACGTLTAGLTVGILLGSVVATLINTSLSPQGIHDGGWRIPFLLGGVFGLIAMYLRRWLQETPIFLEMQQRKALAQELPVKAVALKHQKAVVISMLLTWLLSAGVVVVILMSPVWLQKHYGFAPAITLQANSIATIMLCIGCLLAGLAADRFGASRTFIVGSLLLAVASWAFYHLAGASPQRLFLLYGTVGLCVGVVGAVPYVMVRAFPAEVRFTGISFSYNLSYAIFGGLTPIAVTMLMGVSPMAPAWYVLALSLMGLGLGIWLRQELTAPTGMPEGELQR

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

4
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015293 Enables the active transport of a solute across a membrane by a mechanism whereby two or more species are transported together in the same direction in a tightly coupled process not directly linked to a form of energy other than chemiosmotic energy.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

49 records
Show feature table
Start End DB Term Name
298 308 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
89 109 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
188 207 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
115 141 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
21 43 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
208 242 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
242 264 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
269 273 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
93 115 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
243 268 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
58 80 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
40 58 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
277 299 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
334 355 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
375 394 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
400 417 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
418 434 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
177 187 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
274 297 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
237 423 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
237 423 InterPro IPR036259 MFS transporter superfamily
151 173 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
12 230 FunFam G3DSA:1.20.1250.20:FF:000001 Dicarboxylate MFS transporter
398 417 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
4 419 PANTHER PTHR43528 ALPHA-KETOGLUTARATE PERMEASE
329 333 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
10 236 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
10 236 InterPro IPR036259 MFS transporter superfamily
371 393 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
11 420 SUPERFAMILY SSF103473 MFS general substrate transporter
11 420 InterPro IPR036259 MFS transporter superfamily
304 326 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
336 358 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
15 422 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
15 422 InterPro IPR020846 Major facilitator superfamily domain
59 381 Pfam PF07690 Major Facilitator Superfamily
59 381 InterPro IPR011701 Major facilitator superfamily
153 176 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
309 328 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
395 399 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
356 374 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
120 142 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 15 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
188 207 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
16 39 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
110 114 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
78 88 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
142 152 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
59 77 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.91
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Surrounding area
Pocket 2 P2Rank #2
0.363
Likely same site as FPocket 5 1.7 Å 13 shared residues 100% of smaller site
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Surrounding area
Pocket 3 P2Rank #3
0.329
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Surrounding area
Pocket 4 P2Rank #4
0.176
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Surrounding area
Pocket 5 P2Rank #5
0.036
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #5
0.888
Likely same site as P2Rank 2 1.7 Å 13 shared residues 100% of smaller site
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Surrounding area
Pocket 2 FPocket #29
0.335
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GIY8
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_01985
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GIY8