Protein profile

KP13_02032

Ferric iron reductase protein fhuF

Genome: KpKP13

Gene: fhuF AHE46468.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GRB8
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Amino acids 262
Annotations 2
Features 22
PDB binders 0
Druggability 0.341

Overview

Basic information about this protein and its source genome.

Accession
KP13_02032
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
fhuF AHE46468.1
Status
annotated
Amino acids
262
Structure source
AlphaFold + ColabFold
GO
GO:0051537 Binding to a 2 iron, 2 sulfur (2Fe-2S) cluster; this cluster consists of two iron atoms, with two inorganic sulfur atoms found between the irons and acting as bridging ligands. GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
93.2

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.341
Structure A0A0H3GRB8
Pocket Pocket 3
P2Rank 0.057
Structure A0A0H3GRB8
Pocket Pocket 1
ColabFold model
FPocket 0.7 · Pocket 1
P2Rank 0.232 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 64 / 4744 genomes with a hit
Normalized 0.013

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

2 GO

Gene Ontology (GO)

2
  • GO:0051537 Binding to a 2 iron, 2 sulfur (2Fe-2S) cluster; this cluster consists of two iron atoms, with two inorganic sulfur atoms found between the irons and acting as bridging ligands.
  • GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.

Sequence Features

Domain/signature hits from InterPro and related databases.

22 records
Show feature table
Start End DB Term Name
241 260 Pfam PF11575 FhuF 2Fe-2S C-terminal domain
241 260 InterPro IPR024726 Ferric siderophore reductase, C-terminal
125 139 PRINTS PR01714 Ferric iron reductase signature
125 139 InterPro IPR008090 Ferric iron reductase
93 108 PRINTS PR01714 Ferric iron reductase signature
93 108 InterPro IPR008090 Ferric iron reductase
56 73 PRINTS PR01714 Ferric iron reductase signature
56 73 InterPro IPR008090 Ferric iron reductase
173 189 PRINTS PR01714 Ferric iron reductase signature
173 189 InterPro IPR008090 Ferric iron reductase
197 212 PRINTS PR01714 Ferric iron reductase signature
197 212 InterPro IPR008090 Ferric iron reductase
241 259 PRINTS PR01714 Ferric iron reductase signature
241 259 InterPro IPR008090 Ferric iron reductase
16 32 PRINTS PR01714 Ferric iron reductase signature
16 32 InterPro IPR008090 Ferric iron reductase
215 231 PRINTS PR01714 Ferric iron reductase signature
215 231 InterPro IPR008090 Ferric iron reductase
93 238 Pfam PF06276 Ferric iron reductase FhuF-like transporter
93 238 InterPro IPR022770 Aerobactin siderophore biosynthesis IucA/IucC-like, C-terminal
87 262 NCBIfam TIGR03951 siderophore-iron reductase FhuF
87 262 InterPro IPR008090 Ferric iron reductase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GRB8
AlphaFold — — full sequence — Viewing
ColabFold KP13_02032
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.341
2 0.207

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 1.78 0.033
2 1.35 0.016