Protein profile

KP13_03714

Cation efflux system protein cusB

Genome: KpKP13

Gene: AHE46555.1 cusB Structure source: AlphaFold + ColabFold UniProt A0A0H3GHP7
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Amino acids 420
Annotations 8
Features 19
PDB binders 0
Druggability 0.318

Overview

Basic information about this protein and its source genome.

Accession
KP13_03714
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE46555.1 cusB
Status
annotated
Amino acids
420
Structure source
AlphaFold + ColabFold
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other. GO:0046872 Binding to a metal ion. GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0030288 The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall. GO:0046914 Binding to a transition metal ions; a transition metal is an element whose atom has an incomplete d-subshell of extranuclear electrons, or which gives rise to a cation or cations with an incomplete d-subshell. Transition metals often have more than one valency state. Biologically relevant transition metals include vanadium, manganese, iron, copper, cobalt, nickel, molybdenum and silver.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Unknown
ColabFold pLDDT
81.73

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.318
Structure A0A0H3GHP7
Pocket Pocket 7
P2Rank 0.013
Structure A0A0H3GHP7
Pocket Pocket 1
ColabFold model
FPocket 0.626 · Pocket 7
P2Rank 0.022 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 55 / 4744 genomes with a hit
Normalized 0.012

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

8 GO

Gene Ontology (GO)

8
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0046872 Binding to a metal ion.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0030288 The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall.
  • GO:0046914 Binding to a transition metal ions; a transition metal is an element whose atom has an incomplete d-subshell of extranuclear electrons, or which gives rise to a cation or cations with an incomplete d-subshell. Transition metals often have more than one valency state. Biologically relevant transition metals include vanadium, manganese, iron, copper, cobalt, nickel, molybdenum and silver.
  • GO:0060003 The directed movement of copper ions out of a cell or organelle.
  • GO:0015679 The directed movement of copper ions across the plasma membrane.

Sequence Features

Domain/signature hits from InterPro and related databases.

19 records
Show feature table
Start End DB Term Name
326 420 Gene3D G3DSA:2.40.420.20 -
16 401 PANTHER PTHR30097 CATION EFFLUX SYSTEM PROTEIN CUSB
9 28 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
125 324 SUPERFAMILY SSF111369 HlyD-like secretion proteins
326 419 FunFam G3DSA:2.40.420.20:FF:000003 Cation efflux system protein cusB
1 23 Phobius SIGNAL_PEPTIDE Signal peptide region
229 325 Gene3D G3DSA:2.40.30.170 -
162 212 Gene3D G3DSA:6.10.140.730 -
10 18 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
205 397 NCBIfam TIGR01730 efflux RND transporter periplasmic adaptor subunit
205 397 InterPro IPR006143 RND efflux pump, membrane fusion protein
19 23 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
24 420 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 35 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
115 320 Pfam PF16576 Barrel-sandwich domain of CusB or HlyD membrane-fusion
115 320 InterPro IPR032317 RND efflux pump, membrane fusion protein, barrel-sandwich domain
48 75 Pfam PF19335 Heavy metal binding domain
48 75 InterPro IPR045800 Heavy metal binding domain
1 9 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GHP7
AlphaFold — — full sequence — Viewing
ColabFold KP13_03714
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
7 0.318
5 0.213