Protein profile

KP13_01285

PTS system trehalose-specific EIIBC component

Genome: KpKP13

Gene: treB AHE46649.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GMH8
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Amino acids 472
Annotations 8
Features 53
PDB binders 0
Druggability 0.496

Overview

Basic information about this protein and its source genome.

Accession
KP13_01285
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
treB AHE46649.1
Status
annotated
Amino acids
472
Structure source
AlphaFold + ColabFold
GO
GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0015771 The directed movement of trehalose into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Trehalose is a disaccharide that consists of two molecules of glucose and is isomeric with sucrose. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0015574 Enables the transfer of trehalose from one side of a membrane to the other. Trehalose is the disaccharide alpha-D-glucopyranosyl-alpha-D-glucopyranoside that acts of a reserve carbohydrate in certain fungi, algae and lichens. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
88.64

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.496
Structure A0A0H3GMH8
Pocket Pocket 5
P2Rank 0.924
Structure A0A0H3GMH8
Pocket Pocket 1
ColabFold model
FPocket 0.983 · Pocket 15
P2Rank 0.895 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 256 / 4744 genomes with a hit
Normalized 0.054

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

8 GO

Gene Ontology (GO)

8
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0015771 The directed movement of trehalose into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Trehalose is a disaccharide that consists of two molecules of glucose and is isomeric with sucrose.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0015574 Enables the transfer of trehalose from one side of a membrane to the other. Trehalose is the disaccharide alpha-D-glucopyranosyl-alpha-D-glucopyranoside that acts of a reserve carbohydrate in certain fungi, algae and lichens.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0090589 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + trehalose (out) = protein cysteine + trehalose-6-phosphate (in).

Sequence Features

Domain/signature hits from InterPro and related databases.

53 records
Show feature table
Start End DB Term Name
154 176 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
11 40 Pfam PF00367 phosphotransferase system, EIIB
11 40 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
208 226 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
245 255 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
158 179 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
372 391 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
225 244 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
106 130 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
398 418 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
106 128 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
361 371 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
300 320 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
227 244 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
30 111 NCBIfam TIGR00826 glucose PTS transporter subunit EIIB
30 111 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
108 472 ProSiteProfiles PS51103 PTS_EIIC type-1 domain profile.
108 472 InterPro IPR013013 Phosphotransferase system, EIIC component, type 1
340 360 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
321 339 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
10 81 SUPERFAMILY SSF55604 Glucose permease domain IIB
10 81 InterPro IPR036878 Glucose permease domain IIB
405 427 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
437 459 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
6 460 NCBIfam TIGR01992 PTS system trehalose-specific EIIBC component
6 460 InterPro IPR011296 Phosphotransferase system, trehalose-specific IIBC component
419 437 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
392 397 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
6 88 ProSiteProfiles PS51098 PTS_EIIB type-1 domain profile.
6 88 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
298 320 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
131 157 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
370 392 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
183 205 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
5 82 FunFam G3DSA:3.30.1360.60:FF:000001 PTS system glucose-specific IIBC component PtsG
21 38 ProSitePatterns PS01035 PTS EIIB domains cysteine phosphorylation site signature.
21 38 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
438 460 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
256 280 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
112 404 Pfam PF02378 Phosphotransferase system, EIIC
112 404 InterPro IPR003352 Phosphotransferase system, EIIC
461 472 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
6 83 CDD cd00212 PTS_IIB_glc
6 83 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
1 105 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
5 461 PANTHER PTHR30175 PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN
180 185 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
4 81 Gene3D G3DSA:3.30.1360.60 Glucose permease domain IIB
4 81 InterPro IPR036878 Glucose permease domain IIB
256 278 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
341 360 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
186 207 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
281 299 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GMH8
AlphaFold — — full sequence — Viewing
ColabFold KP13_01285
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
16 0.46
19 0.182
1 0.002
25 0.002

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 34.07 0.924
2 5.85 0.236
3 4.7 0.17
4 2.67 0.064
5 2.61 0.061