KpKP13 Protein target profile

Phosphotransferase system, lactose/cellobiose IIC component

Accession: KP13_01283

Gene: AHE46651.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GHH9
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Length 450
Pocket druggability (P2Rank · AlphaFold DB model) 0.709
Functional annotation 0 EC 5 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Independent pocket support: P2Rank + FPocket Both predictors report a high-scoring binding site (P2Rank 0.71, FPocket 0.97). Use the 3D pocket comparison to verify whether they identify the same site. No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Strong predicted binding pocket FPocket selected-pocket score 0.97. This is a computational pocket signal, not binding validation. Review the complete evidence below

Risks to review

Microbiome similarity 21 of 4744 screened genomes (0.4%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.71) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
0.4% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
91.35 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.709
Structure A0A0H3GHH9
Pocket Pocket 1
Druggability (FPocket) 0.974
Structure A0A0H3GHH9
Pocket Pocket 10
ColabFold model
P2Rank 0.692 · Pocket 1
FPocket 0.803 · Pocket 3
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 21 / 4744 genomes with a hit
Prevalence 0.4%

Sequence

Primary amino-acid sequence viewer.

MSNTSWIERYVMPAALRIAGQKHVLSVRDGIILNMPFMLIGSFFLIFAYLPIPGYADMMSSLFGEVWRDKMLYPVKATYDIMALISSFGIAYRLAEKYRTLDPLSAGAMSLVAFMMTIPQNTLFTPVHGAAEVIKGVIPVSMVGSQGLFVAIVISLLSTEIYRLVASRNLVIRMPDGVPPAVAKSFLALIPGFCVLAVVLALCLAVEASPFGDINSMIATLIGIPMHHVGGTLPGMIISVILIGILWTLGLHGDAIVLVFIQPVWLSNMSENLTAFQNGQPIPHIITQQFYDLWIAPGGTGALLGLVIFMLLRSRSQQMKQLGKIAAPGALFNISEPMVFGIPLVMNPYFFLPFILTPVLLVIVSYTAMATGLVAPPAGIALPFTTPIFISGYLATGGHISGTVLQVVNLAISLVVYYPFFRAWDRLKAKEEHASAQPQASAAIADADRA

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

5
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:1901264 The directed movement of a carbohydrate derivative into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

46 records
Show feature table
Start End DB Term Name
93 103 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
314 324 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
186 208 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
349 366 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
399 421 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
421 450 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
240 261 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
373 394 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
290 312 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
344 348 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
186 208 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
7 420 ProSiteProfiles PS51105 PTS_EIIC type-3 domain profile.
7 420 InterPro IPR004501 Phosphotransferase system, EIIC component, type 3
367 372 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
106 128 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
325 343 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
373 395 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
228 250 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
53 71 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
143 165 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
234 239 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
28 358 Pfam PF02378 Phosphotransferase system, EIIC
28 358 InterPro IPR003352 Phosphotransferase system, EIIC
5 438 PANTHER PTHR33989 -
209 213 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
31 52 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
262 292 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
11 433 NCBIfam TIGR00359 PTS cellobiose transporter subunit IIC
11 433 InterPro IPR004796 Phosphotransferase system, cellobiose-type IIC component
124 142 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
344 366 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
143 165 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
395 399 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
30 52 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
11 433 NCBIfam TIGR00410 PTS system, lactose/cellobiose family IIC component
11 433 InterPro IPR004501 Phosphotransferase system, EIIC component, type 3
72 92 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
214 233 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 30 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
166 185 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
293 313 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
400 420 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
104 123 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
2 443 PIRSF PIRSF006351 PTS_EIIC-Cellobiose
2 443 InterPro IPR004796 Phosphotransferase system, cellobiose-type IIC component
72 94 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.709
Likely same site as FPocket 5 1.7 Å 17 shared residues 100% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.238
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Surrounding area
Pocket 3 P2Rank #3
0.138
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Surrounding area
Pocket 4 P2Rank #4
0.051
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Surrounding area
Pocket 5 P2Rank #5
0.033
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #10
0.974 Unusual size
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Surrounding area
Pocket 2 FPocket #2
0.901
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Surrounding area
Pocket 3 FPocket #5
0.236
Likely same site as P2Rank 1 1.7 Å 17 shared residues 100% of smaller site
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GHH9
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_01283
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GHH9