Protein profile

KP13_01274

phosphoenolpyruvate-dependent sugar phosphotransferase system EIIA 2

Genome: KpKP13

Gene: AHE46660.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GHH1
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Amino acids 636
Annotations 5
Features 37
PDB binders 0
Druggability 0.376

Overview

Basic information about this protein and its source genome.

Accession
KP13_01274
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE46660.1
Status
annotated
Amino acids
636
Structure source
AlphaFold + ColabFold
GO
GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription. GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons. GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
89.51

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.376
Structure A0A0H3GHH1
Pocket Pocket 3
P2Rank 0.591
Structure A0A0H3GHH1
Pocket Pocket 1
ColabFold model
FPocket 0.968 · Pocket 22
P2Rank 0.526 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 83 / 4744 genomes with a hit
Normalized 0.017

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
  • GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons.
  • GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).

Sequence Features

Domain/signature hits from InterPro and related databases.

37 records
Show feature table
Start End DB Term Name
401 483 CDD cd05568 PTS_IIB_bgl_like
300 386 Pfam PF00874 PRD domain
300 386 InterPro IPR011608 PRD domain
1 7 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
87 165 Pfam PF05043 Mga helix-turn-helix domain
87 165 InterPro IPR007737 Mga helix-turn-helix domain
294 387 Gene3D G3DSA:1.10.1790.10 PRD domain
7 63 Pfam PF08279 HTH domain
7 63 InterPro IPR013196 Helix-turn-helix, type 11
2 65 Gene3D G3DSA:1.10.10.10 -
2 65 InterPro IPR036388 Winged helix-like DNA-binding domain superfamily
288 395 ProSiteProfiles PS51372 PRD domain profile.
288 395 InterPro IPR011608 PRD domain
293 391 SUPERFAMILY SSF63520 PTS-regulatory domain, PRD
293 391 InterPro IPR036634 PRD domain superfamily
398 484 Gene3D G3DSA:3.40.50.2300 -
509 625 Pfam PF00359 Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2
509 625 InterPro IPR002178 PTS EIIA type-2 domain
8 15 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
401 478 SUPERFAMILY SSF52794 PTS system IIB component-like
401 478 InterPro IPR036095 PTS system IIB component-like superfamily
1 21 Phobius SIGNAL_PEPTIDE Signal peptide region
494 632 ProSiteProfiles PS51094 PTS_EIIA type-2 domain profile.
494 632 InterPro IPR002178 PTS EIIA type-2 domain
16 21 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
401 490 ProSiteProfiles PS51099 PTS_EIIB type-2 domain profile.
401 490 InterPro IPR013011 Phosphotransferase system, EIIB component, type 2
496 628 CDD cd00211 PTS_IIA_fru
496 628 InterPro IPR002178 PTS EIIA type-2 domain
490 629 Gene3D G3DSA:3.40.930.10 -
490 629 InterPro IPR016152 Phosphotransferase/anion transporter
22 636 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
490 627 SUPERFAMILY SSF55804 Phoshotransferase/anion transport protein
490 627 InterPro IPR016152 Phosphotransferase/anion transporter
7 40 ProSitePatterns PS00894 DeoR-type HTH domain signature.
7 40 InterPro IPR018356 Transcription regulator, HTH DeoR-type, conserved site
4 628 PANTHER PTHR30185 CRYPTIC BETA-GLUCOSIDE BGL OPERON ANTITERMINATOR

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GHH1
AlphaFold — — full sequence — Viewing
ColabFold KP13_01274
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.376
5 0.277

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 8.7 0.465
2 5.36 0.254
3 4.23 0.173
4 4.18 0.17
5 3.03 0.1