Protein target profile

KP13_03528

putative transposase InsK for insertion sequence element IS150

Genome: KpKP13 Gene: insK AHE46674.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GRH9
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Length 252
Pocket druggability 0.019
Functional annotation 0 EC 2 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Structure available AlphaFold DB model + ColabFold model

Risks to review

Microbiome similarity 195 of 4744 screened genomes (4.1%) have a significant similarity hit. Mixed conservation Core/accessory calls are not fully concordant. Weak predicted binding pocket FPocket selected-pocket score 0.02. Weak second pocket-predictor signal P2Rank predicted binding-site probability 0.24.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
4.1% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
51.639 Higher values support similarity to known essential genes.
DEG E-value
2.5999999999999996e-90 Smaller values mean stronger essential-gene similarity.

Localization

Localization
Unknown

Structure confidence

ColabFold pLDDT
88.33 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

The selected pocket score is the FPocket value used for ranking after applying the curated structure priority. It estimates small-molecule pocket quality; it is not experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

FPocket 0.019
Structure A0A0H3GRH9
Pocket Pocket 2
P2Rank 0.236
Structure A0A0H3GRH9
Pocket Pocket 1
ColabFold model
FPocket 0.214 · Pocket 10
P2Rank 0.644 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 195 / 4744 genomes with a hit
Prevalence 4.1%

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GRH9

Sequence

Primary amino-acid sequence viewer.

MNALKQGDRYAGLKENIRKIYHYHKGRYGYRRITLALRKQGLRINHKTVQRLMAELSLRSVIRAKKYRAWKGRTGEAAPNILSRNFGASKANEKWVTDVTEFPVQGKKLYLSSVLDLFNREVIAYSLSERPVMEMVNTMLDGAFPKLRPGDAPLLHSDQGWHYRMRSYQERLKAHGMTQSMSRKGNCLDNAVMENFFGTLKSECFYLREFRSVSALRKAVEDYIHYYNNERISLKLKGLSPVEYRTQALRAA

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

2 GO

Gene Ontology (GO)

2
  • GO:0003676 Binding to a nucleic acid.
  • GO:0015074 The process in which a DNA segment is incorporated into another, usually larger, DNA molecule such as a chromosome.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

14 records
Show feature table
Start End DB Term Name
84 243 SUPERFAMILY SSF53098 Ribonuclease H-like
84 243 InterPro IPR012337 Ribonuclease H-like superfamily
75 249 ProSiteProfiles PS50994 Integrase catalytic domain profile.
75 249 InterPro IPR001584 Integrase, catalytic core
194 249 Pfam PF13333 Integrase core domain
194 249 InterPro IPR001584 Integrase, catalytic core
8 245 NCBIfam NF033516 IS3 family transposase
13 66 Pfam PF13276 HTH-like domain
13 66 InterPro IPR025948 HTH-like domain
90 187 Pfam PF00665 Integrase core domain
90 187 InterPro IPR001584 Integrase, catalytic core
12 247 PANTHER PTHR46889 TRANSPOSASE INSF FOR INSERTION SEQUENCE IS3B-RELATED
85 245 Gene3D G3DSA:3.30.420.10 -
85 245 InterPro IPR036397 Ribonuclease H superfamily

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Probability: high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Site 1 P2Rank #1
0.236
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Surrounding area
Site 2 P2Rank #2
0.149
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Surrounding area
Site 3 P2Rank #3
0.118
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Surrounding area
Site 4 P2Rank #4
0.025
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Surrounding area
Site 5 P2Rank #5
0.024
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GRH9
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_03528
ColabFold — — full sequence — Loaded