KpKP13 Protein target profile

Lactose permease

Accession: KP13_01242

Gene: AHE46694.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A9J6S0U4
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Length 410
Pocket druggability (P2Rank · AlphaFold DB model) 0.959
Functional annotation 0 EC 3 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Second pocket predictor agrees P2Rank predicted binding-site probability 0.96. Structure available AlphaFold DB model + ColabFold model

Risks to review

Microbiome similarity 9 of 4744 screened genomes (0.2%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.96) — check the ligand evidence before deprioritizing on off-target risk alone. Mixed conservation Core/accessory calls are not fully concordant. Weak predicted binding pocket FPocket selected-pocket score 0.17.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
0.2% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
42.593 Higher values support similarity to known essential genes.
DEG E-value
7.29e-106 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
83.04 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.959
Structure A0A9J6S0U4
Pocket Pocket 1
Druggability (FPocket) 0.173
Structure A0A9J6S0U4
Pocket Pocket 26
ColabFold model
P2Rank 0.974 · Pocket 1
FPocket 0.762 · Pocket 15
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 9 / 4744 genomes with a hit
Prevalence 0.2%

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A9J6S0U4

Sequence

Primary amino-acid sequence viewer.

MITSNKNYFISCLFFLFFFIGWGCCYPYLSLWLTETIGINYTDVGLVYSFTAIVAVCVQPLFGFISDKLVYRKNLMWMLAIIITLFAPYWIYVFAPLLKINVFLGALAGGLYIGMAYGAGCGVCEAYIDKVSRASGFEFGRARMFGGIGAAIGTFAAGKLYGIDQNMIFWLASGAGVCLLVIVWKMQISAHPQQGLLSGKASPVTLRDAVSLLKIKKFWFFALYVIGVGAVYETYDQQFAIYYSHFFESKARGAEVFGYLTTGQIFLDAIVMFFAPWFVNKIGPKNALLYCGLIMSLRIIGSAWAIGPVSISLIKLLHGFESSVLLVAALKYITANFNPLLSATVYLIGFQFSKSFSSIFLSTGIGHMYQSMGFTSSYIVLGGIALCFTLISFITLDKARVFSSQPVPAN

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

3
  • GO:0008643 The directed movement of carbohydrate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Carbohydrates are a group of organic compounds based of the general formula Cx(H2O)y.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0005351 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: carbohydrate(out) + H+(out) = carbohydrate(in) + H+(in).

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

63 records
Show feature table
Start End DB Term Name
2 194 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
2 194 InterPro IPR036259 MFS transporter superfamily
34 44 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
185 217 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
202 407 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
202 407 InterPro IPR036259 MFS transporter superfamily
345 369 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
162 166 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
7 29 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
220 242 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
66 76 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
144 163 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
98 102 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
313 333 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
370 374 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
167 184 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
44 66 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
5 399 SUPERFAMILY SSF103473 MFS general substrate transporter
5 399 InterPro IPR036259 MFS transporter superfamily
218 236 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
237 255 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
4 402 Pfam PF01306 LacY proton/sugar symporter
4 402 InterPro IPR000576 LacY/RafB permease family
256 275 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
334 344 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
129 139 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
45 65 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
4 400 PANTHER PTHR23522 BLL5896 PROTEIN
77 97 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
286 308 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
287 307 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
308 312 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
328 350 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
210 238 PRINTS PR00174 LacY proton/sugar symporter family signature
210 238 InterPro IPR000576 LacY/RafB permease family
256 277 PRINTS PR00174 LacY proton/sugar symporter family signature
256 277 InterPro IPR000576 LacY/RafB permease family
280 306 PRINTS PR00174 LacY proton/sugar symporter family signature
280 306 InterPro IPR000576 LacY/RafB permease family
342 362 PRINTS PR00174 LacY proton/sugar symporter family signature
342 362 InterPro IPR000576 LacY/RafB permease family
144 162 PRINTS PR00174 LacY proton/sugar symporter family signature
144 162 InterPro IPR000576 LacY/RafB permease family
102 124 PRINTS PR00174 LacY proton/sugar symporter family signature
102 124 InterPro IPR000576 LacY/RafB permease family
67 96 PRINTS PR00174 LacY proton/sugar symporter family signature
67 96 InterPro IPR000576 LacY/RafB permease family
311 330 PRINTS PR00174 LacY proton/sugar symporter family signature
311 330 InterPro IPR000576 LacY/RafB permease family
167 184 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
257 279 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
102 124 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
276 286 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
397 410 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
75 97 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
103 128 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
375 396 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
140 161 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
5 396 NCBIfam TIGR00882 oligosaccharide MFS transporter
5 396 InterPro IPR000576 LacY/RafB permease family
374 396 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
12 33 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.959
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Surrounding area
Pocket 2 P2Rank #2
0.12
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Surrounding area
Pocket 3 P2Rank #3
0.022
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Surrounding area
Pocket 4 P2Rank #4
0.02
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Surrounding area
Pocket 5 P2Rank #5
0.009
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A9J6S0U4
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_01242
ColabFold — — full sequence — Loaded