Protein profile

KP13_32181

HTH-type transcriptional repressor NsrR

Genome: KpKP13

Gene: AHE46731.1 nsrR Structure source: AlphaFold + ColabFold UniProt A0A0H3GMB2
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Amino acids 156
Annotations 6
Features 17
PDB binders 1
Druggability 0.227

Overview

Basic information about this protein and its source genome.

Accession
KP13_32181
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE46731.1 nsrR
Status
annotated
Amino acids
156
Structure source
AlphaFold + ColabFold
GO
GO:0045892 Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-templated transcription. GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0051537 Binding to a 2 iron, 2 sulfur (2Fe-2S) cluster; this cluster consists of two iron atoms, with two inorganic sulfur atoms found between the irons and acting as bridging ligands. GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons. GO:0003690 Binding to double-stranded DNA. GO:0005506 Binding to an iron (Fe) ion.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
89.5

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.227
Structure A0A0H3GMB2
Pocket Pocket 1
P2Rank 0.008
Structure A0A0H3GMB2
Pocket Pocket 1
ColabFold model
FPocket 0.409 · Pocket 7
P2Rank 0.005 · Pocket 1
Core conservation Accessory gene
Roary core
CoreCruncher accessory
Gut microbiome 116 / 4744 genomes with a hit
Normalized 0.024

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0045892 Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-templated transcription.
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0051537 Binding to a 2 iron, 2 sulfur (2Fe-2S) cluster; this cluster consists of two iron atoms, with two inorganic sulfur atoms found between the irons and acting as bridging ligands.
  • GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons.
  • GO:0003690 Binding to double-stranded DNA.
  • GO:0005506 Binding to an iron (Fe) ion.

Sequence Features

Domain/signature hits from InterPro and related databases.

17 records
Show feature table
Start End DB Term Name
16 156 Hamap MF_01177 HTH-type transcriptional repressor NsrR [nsrR].
16 156 InterPro IPR023761 Transcriptional repressor HTH, NsrR
16 149 Gene3D G3DSA:1.10.10.10 -
16 149 InterPro IPR036388 Winged helix-like DNA-binding domain superfamily
64 82 ProSitePatterns PS01332 Rrf2-type HTH domain signature.
64 82 InterPro IPR030489 Transcription regulator Rrf2-type, conserved site
18 144 Pfam PF02082 Iron-dependent Transcriptional regulator
18 144 InterPro IPR000944 Transcription regulator Rrf2
17 146 SUPERFAMILY SSF46785 Winged helix DNA-binding domain
17 146 InterPro IPR036390 Winged helix DNA-binding domain superfamily
17 143 NCBIfam TIGR00738 Rrf2 family transcriptional regulator
17 143 InterPro IPR000944 Transcription regulator Rrf2
17 151 PANTHER PTHR33221 WINGED HELIX-TURN-HELIX TRANSCRIPTIONAL REGULATOR, RRF2 FAMILY
17 151 InterPro IPR000944 Transcription regulator Rrf2
16 149 FunFam G3DSA:1.10.10.10:FF:000105 HTH-type transcriptional repressor NsrR
17 144 ProSiteProfiles PS51197 Rrf2-type HTH domain profile.
17 144 InterPro IPR000944 Transcription regulator Rrf2

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GMB2
AlphaFold — — full sequence — Viewing
ColabFold KP13_32181
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.227

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 1.09 0.007

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

1 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
FES
6HSD
F2RGC9 175.8 Da LogP 1.29 TPSA 0.0 ✓ Ro5 ✓ Clean S1[Fe]S[Fe]1

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.