Protein profile

KP13_00532

Protein hflK

Genome: KpKP13

Gene: hflK AHE46736.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GLC9
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Amino acids 420
Annotations 3
Features 31
PDB binders 0
Druggability 0.427

Overview

Basic information about this protein and its source genome.

Accession
KP13_00532
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
hflK AHE46736.1
Status
annotated
Amino acids
420
Structure source
AlphaFold + ColabFold
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0008233 Catalysis of the hydrolysis of a peptide bond. A peptide bond is a covalent bond formed when the carbon atom from the carboxyl group of one amino acid shares electrons with the nitrogen atom from the amino group of a second amino acid. GO:0006508 The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
25.0
Human E-value
6.71e-06
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
88.836
DEG E-value
0.0
Localization
Unknown
ColabFold pLDDT
74.63

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.427
Structure A0A0H3GLC9
Pocket Pocket 29
P2Rank
Structure A0A0H3GLC9
Pocket No pockets
ColabFold model
FPocket 0.55 · Pocket 2
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 144 / 4744 genomes with a hit
Normalized 0.03

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Gene Ontology (GO)

3
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008233 Catalysis of the hydrolysis of a peptide bond. A peptide bond is a covalent bond formed when the carbon atom from the carboxyl group of one amino acid shares electrons with the nitrogen atom from the amino group of a second amino acid.
  • GO:0006508 The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.

Sequence Features

Domain/signature hits from InterPro and related databases.

31 records
Show feature table
Start End DB Term Name
253 273 Coils Coil Coil
101 123 PRINTS PR00721 Stomatin signature
101 123 InterPro IPR001972 Stomatin/HflK family
148 169 PRINTS PR00721 Stomatin signature
148 169 InterPro IPR001972 Stomatin/HflK family
183 200 PRINTS PR00721 Stomatin signature
183 200 InterPro IPR001972 Stomatin/HflK family
1 78 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
125 256 Gene3D G3DSA:3.30.479.30 Band 7 domain
125 256 InterPro IPR036013 Band 7/SPFH domain superfamily
77 99 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 45 MobiDBLite mobidb-lite consensus disorder prediction
94 254 SMART SM00244 PHB_4
94 254 InterPro IPR001107 Band 7 domain
366 420 MobiDBLite mobidb-lite consensus disorder prediction
94 340 CDD cd03404 SPFH_HflK
94 340 InterPro IPR010201 HflK
127 258 SUPERFAMILY SSF117892 Band 7/SPFH domain
127 258 InterPro IPR036013 Band 7/SPFH domain superfamily
125 256 FunFam G3DSA:3.30.479.30:FF:000007 Protein HflK
96 356 NCBIfam TIGR01933 FtsH protease activity modulator HflK
96 356 InterPro IPR010201 HflK
378 420 MobiDBLite mobidb-lite consensus disorder prediction
79 99 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
100 420 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 15 MobiDBLite mobidb-lite consensus disorder prediction
98 269 Pfam PF01145 SPFH domain / Band 7 family
98 269 InterPro IPR001107 Band 7 domain
93 347 PANTHER PTHR43327 STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL
1 59 Pfam PF12221 Bacterial membrane protein N terminal
1 59 InterPro IPR020980 Menbrane protein HflK, N-terminal

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GLC9
AlphaFold — — full sequence — Viewing
ColabFold KP13_00532
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
29 0.427
7 0.27
23 0.216

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

6 records

Bioactivity inferred from similar proteins in ChEMBL. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

Show only:
Ligand UniProt (homolog) pchembl MW · LogP · TPSA Lipinski PAINS SMILES
DWT P27105 7.72 503.5 Da LogP 5.75 TPSA 97.6 2 viol. ✓ Clean Cc1ccc(cc1Nc2c3cn(nc3nc(n2)c4cccnc4)C)C(=O)Nc5c…
DXH Q9UJZ1 7.70 503.5 Da LogP 5.75 TPSA 97.6 2 viol. ✓ Clean Cc1ccc(cc1Nc2c3cnn(c3nc(n2)c4cccnc4)C)C(=O)Nc5c…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.