Protein profile

KP13_00524

epoxyqueosine reductase

Genome: KpKP13

Gene: queG AHE46742.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GM97
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Amino acids 379
Annotations 9
Features 16
PDB binders 0
Druggability 0.283

Overview

Basic information about this protein and its source genome.

Accession
KP13_00524
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
queG AHE46742.1
Status
annotated
Amino acids
379
Structure source
AlphaFold + ColabFold
EC
1.17.99.6
GO
GO:0016491 Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced. GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group. GO:0008616 The chemical reactions and pathways resulting in the formation of queuosines, a series of nucleosides found in position 34 of tRNA and having an additional pentenyl ring added via an NH group to the methyl group of 7-methylguanosine. The pentenyl ring may carry other substituents. The wobble nucleoside of the tRNA sequence (position 34) corresponds to the first position of the anticodon. GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0051539 Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. GO:0031419 Binding to cobalamin (vitamin B12), a water-soluble vitamin characterized by possession of a corrin nucleus containing a cobalt atom.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
94.54

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.283
Structure A0A0H3GM97
Pocket Pocket 18
P2Rank 0.969
Structure A0A0H3GM97
Pocket Pocket 1
ColabFold model
FPocket 0.604 · Pocket 20
P2Rank 0.967 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 153 / 4744 genomes with a hit
Normalized 0.032

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0016491 Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
  • GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
  • GO:0008616 The chemical reactions and pathways resulting in the formation of queuosines, a series of nucleosides found in position 34 of tRNA and having an additional pentenyl ring added via an NH group to the methyl group of 7-methylguanosine. The pentenyl ring may carry other substituents. The wobble nucleoside of the tRNA sequence (position 34) corresponds to the first position of the anticodon.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0051539 Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
  • GO:0031419 Binding to cobalamin (vitamin B12), a water-soluble vitamin characterized by possession of a corrin nucleus containing a cobalt atom.
  • GO:0052693 Catalysis of the reaction: epoxyqueuosine in tRNA + reductant = queuosine in tRNA + oxidised reductant.
  • GO:0046872 Binding to a metal ion.

Sequence Features

Domain/signature hits from InterPro and related databases.

16 records
Show feature table
Start End DB Term Name
193 256 Pfam PF13484 4Fe-4S double cluster binding domain
14 348 NCBIfam TIGR00276 tRNA epoxyqueuosine(34) reductase QueG
14 348 InterPro IPR004453 Epoxyqueuosine reductase QueG
139 256 SUPERFAMILY SSF46548 alpha-helical ferredoxin
7 315 Hamap MF_00916 Epoxyqueuosine reductase [queG].
7 315 InterPro IPR004453 Epoxyqueuosine reductase QueG
193 204 ProSitePatterns PS00198 4Fe-4S ferredoxin-type iron-sulfur binding region signature.
193 204 InterPro IPR017900 4Fe-4S ferredoxin, iron-sulphur binding, conserved site
62 140 Pfam PF08331 Epoxyqueuosine reductase QueG, DUF1730
62 140 InterPro IPR013542 Epoxyqueuosine reductase QueG, DUF1730
181 213 ProSiteProfiles PS51379 4Fe-4S ferredoxin-type iron-sulfur binding domain profile.
181 213 InterPro IPR017896 4Fe-4S ferredoxin-type, iron-sulphur binding domain
188 258 Gene3D G3DSA:3.30.70.20 -
5 346 PANTHER PTHR30002 EPOXYQUEUOSINE REDUCTASE
5 346 InterPro IPR004453 Epoxyqueuosine reductase QueG
188 258 FunFam G3DSA:3.30.70.20:FF:000017 Epoxyqueuosine reductase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GM97
AlphaFold — — full sequence — Viewing
ColabFold KP13_00524
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
18 0.283

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 37.28 0.957
2 2.51 0.07
3 1.01 0.006