Protein profile

KP13_00395

UvrABC system protein A

Genome: KpKP13

Gene: AHE46867.1 uvrA Structure source: AlphaFold + ColabFold UniProt A0A0H3GGW8
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Amino acids 941
Annotations 9
Features 38
PDB binders 0
Druggability 0.839

Overview

Basic information about this protein and its source genome.

Accession
KP13_00395
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE46867.1 uvrA
Status
annotated
Amino acids
941
Structure source
AlphaFold + ColabFold
GO
GO:0016887 Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient. GO:0006289 A DNA repair process in which a small region of the strand surrounding the damage is removed from the DNA helix as an oligonucleotide. The small gap left in the DNA helix is filled in by the sequential action of DNA polymerase and DNA ligase. Nucleotide excision repair recognizes a wide range of substrates, including damage caused by UV irradiation (pyrimidine dimers and 6-4 photoproducts) and chemicals (intrastrand cross-links and bulky adducts). GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0009380 Any of the protein complexes formed by the UvrABC excinuclease system, which carries out nucleotide excision repair. Three different complexes are formed by the 3 proteins as they proceed through the excision repair process. First a complex consisting of two A subunits and two B subunits bind DNA and unwind it around the damaged site. Then, the A subunits disassociate leaving behind a stable complex between B subunits and DNA. Now, subunit C binds to this B+DNA complex and causes subunit B to nick the DNA on one side of the complex while subunit C nicks the DNA on the other side of the complex. DNA polymerase I and DNA ligase can then repair the resulting gap. GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
32.941
Human E-value
1.45e-06
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
57.827
DEG E-value
0.0
Localization
Cytoplasmic
ColabFold pLDDT
91.17

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.839
Structure A0A0H3GGW8
Pocket Pocket 42
P2Rank 0.946
Structure A0A0H3GGW8
Pocket Pocket 1
ColabFold model
FPocket 0.464 · Pocket 43
P2Rank 0.906 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 3549 / 4744 genomes with a hit
Normalized 0.748

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

9 GO

Gene Ontology (GO)

9
  • GO:0016887 Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
  • GO:0006289 A DNA repair process in which a small region of the strand surrounding the damage is removed from the DNA helix as an oligonucleotide. The small gap left in the DNA helix is filled in by the sequential action of DNA polymerase and DNA ligase. Nucleotide excision repair recognizes a wide range of substrates, including damage caused by UV irradiation (pyrimidine dimers and 6-4 photoproducts) and chemicals (intrastrand cross-links and bulky adducts).
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0009380 Any of the protein complexes formed by the UvrABC excinuclease system, which carries out nucleotide excision repair. Three different complexes are formed by the 3 proteins as they proceed through the excision repair process. First a complex consisting of two A subunits and two B subunits bind DNA and unwind it around the damaged site. Then, the A subunits disassociate leaving behind a stable complex between B subunits and DNA. Now, subunit C binds to this B+DNA complex and causes subunit B to nick the DNA on one side of the complex while subunit C nicks the DNA on the other side of the complex. DNA polymerase I and DNA ligase can then repair the resulting gap.
  • GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0009381 Catalysis of the hydrolysis of ester linkages within deoxyribonucleic acid at sites flanking regions of damaged DNA to which the Uvr ABC excinuclease complexes bind.
  • GO:0008270 Binding to a zinc ion (Zn).
  • GO:0009432 An error-prone process for repairing damaged microbial DNA.

Sequence Features

Domain/signature hits from InterPro and related databases.

38 records
Show feature table
Start End DB Term Name
4 116 CDD cd03270 ABC_UvrA_I
260 487 Gene3D G3DSA:1.20.1580.10 ABC transporter ATPase like domain
290 399 Pfam PF17755 UvrA DNA-binding domain
290 399 InterPro IPR041552 UvrA DNA-binding domain
3 925 NCBIfam TIGR00630 excinuclease ABC subunit UvrA
3 925 InterPro IPR004602 UvrABC system subunit A
613 911 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases
613 911 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
1 940 Hamap MF_00205 UvrABC system protein A [uvrA].
1 940 InterPro IPR004602 UvrABC system subunit A
693 830 FunFam G3DSA:1.20.1580.10:FF:000002 UvrABC system protein A
607 937 ProSiteProfiles PS50893 ATP-binding cassette, ABC transporter-type domain profile.
607 937 InterPro IPR003439 ABC transporter-like, ATP-binding domain
135 244 FunFam G3DSA:3.30.190.20:FF:000003 UvrABC system protein A
2 940 PANTHER PTHR43152 UVRABC SYSTEM PROTEIN A
5 90 Gene3D G3DSA:3.40.50.300 -
5 90 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
488 592 Gene3D G3DSA:3.40.50.300 -
488 592 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
610 937 Gene3D G3DSA:3.40.50.300 -
610 937 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
4 569 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases
4 569 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
806 860 Pfam PF00005 ABC transporter
806 860 InterPro IPR003439 ABC transporter-like, ATP-binding domain
613 921 CDD cd03271 ABC_UvrA_II
487 501 ProSitePatterns PS00211 ABC transporters family signature.
487 501 InterPro IPR017871 ABC transporter-like, conserved site
830 844 ProSitePatterns PS00211 ABC transporters family signature.
830 844 InterPro IPR017871 ABC transporter-like, conserved site
389 487 FunFam G3DSA:1.20.1580.10:FF:000003 UvrABC system protein A
287 404 Gene3D G3DSA:1.10.8.280 -
135 242 Gene3D G3DSA:3.30.190.20 -
693 830 Gene3D G3DSA:1.20.1580.10 ABC transporter ATPase like domain
91 119 Gene3D G3DSA:1.20.1580.10 ABC transporter ATPase like domain
287 404 FunFam G3DSA:1.10.8.280:FF:000001 UvrABC system protein A
130 237 Pfam PF17760 UvrA interaction domain
130 237 InterPro IPR041102 UvrA, interaction domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GGW8
AlphaFold — — full sequence — Viewing
ColabFold KP13_00395
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
42 0.839

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 21.13 0.866
2 19.29 0.837
3 5.74 0.279
4 3.28 0.115
5 2.72 0.081