Protein profile

KP13_08655

tRNA-dihydrouridine synthase A

Genome: KpKP13

Gene: ANJ86641.1 dusA Structure source: AlphaFold + ColabFold UniProt A0A0H3GKY9
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Amino acids 331
Annotations 9
Features 20
PDB binders 0
Druggability 0.416

Overview

Basic information about this protein and its source genome.

Accession
KP13_08655
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
ANJ86641.1 dusA
Status
annotated
Amino acids
331
Structure source
AlphaFold + ColabFold
EC
1.3.1.91
GO
GO:0017150 Catalysis of the reaction: a 5,6-dihydrouridine in tRNA + NAD(P)+ = a uridine in tRNA + H+ + NAD(P)H. GO:0002943 The process whereby a uridine in a transfer RNA is converted to dihydrouridine. GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group. GO:0050660 Binding to FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2. GO:0010181 Binding to flavin mono nucleotide. Flavin mono nucleotide (FMN) is the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes. GO:0000049 Binding to a transfer RNA.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
32.099
Human E-value
5.81e-08
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
67.89
DEG E-value
6.61e-165
Localization
Cytoplasmic
ColabFold pLDDT
94.5

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.416
Structure A0A0H3GKY9
Pocket Pocket 10
P2Rank 0.945
Structure A0A0H3GKY9
Pocket Pocket 1
ColabFold model
FPocket 0.364 · Pocket 5
P2Rank 0.94 · Pocket 1
Core conservation Accessory gene
Roary core
CoreCruncher accessory
Gut microbiome 271 / 4744 genomes with a hit
Normalized 0.057

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0017150 Catalysis of the reaction: a 5,6-dihydrouridine in tRNA + NAD(P)+ = a uridine in tRNA + H+ + NAD(P)H.
  • GO:0002943 The process whereby a uridine in a transfer RNA is converted to dihydrouridine.
  • GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
  • GO:0050660 Binding to FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
  • GO:0010181 Binding to flavin mono nucleotide. Flavin mono nucleotide (FMN) is the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes.
  • GO:0000049 Binding to a transfer RNA.
  • GO:0102264 Catalysis of the reaction: a 5,6-dihydrouracil20 in tRNA + NAD(P) = H+ + a uracil20 in tRNA + NAD(P)H.
  • GO:0102266 Catalysis of the reaction: a 5,6-dihydrouracil20a in tRNA + NAD(P) = H+ + a uracil20a in tRNA + NAD(P)H.

Sequence Features

Domain/signature hits from InterPro and related databases.

20 records
Show feature table
Start End DB Term Name
258 330 Gene3D G3DSA:1.20.120.1460 -
13 252 CDD cd02801 DUS_like_FMN
13 252 InterPro IPR035587 DUS-like, FMN-binding domain
257 330 FunFam G3DSA:1.20.120.1460:FF:000001 tRNA-dihydrouridine(20/20a) synthase
3 326 PIRSF PIRSF006621 Dus
3 326 InterPro IPR001269 tRNA-dihydrouridine synthase
13 328 NCBIfam TIGR00742 tRNA dihydrouridine(20/20a) synthase DusA
13 328 InterPro IPR004653 tRNA-dihydrouridine(20/20a) synthase
12 313 SUPERFAMILY SSF51395 FMN-linked oxidoreductases
8 329 Hamap MF_02041 tRNA-dihydrouridine(20/20a) synthase [dusA].
8 329 InterPro IPR004653 tRNA-dihydrouridine(20/20a) synthase
11 330 PANTHER PTHR42907 FMN-LINKED OXIDOREDUCTASES SUPERFAMILY PROTEIN
11 330 InterPro IPR004653 tRNA-dihydrouridine(20/20a) synthase
11 252 FunFam G3DSA:3.20.20.70:FF:000083 tRNA-dihydrouridine(20/20a) synthase
94 112 ProSitePatterns PS01136 Uncharacterized protein family UPF0034 signature.
94 112 InterPro IPR018517 tRNA-dihydrouridine synthase, conserved site
11 252 Gene3D G3DSA:3.20.20.70 Aldolase class I
11 252 InterPro IPR013785 Aldolase-type TIM barrel
16 325 Pfam PF01207 Dihydrouridine synthase (Dus)
16 325 InterPro IPR035587 DUS-like, FMN-binding domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GKY9
AlphaFold — — full sequence — Viewing
ColabFold KP13_08655
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
10 0.416

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 25.33 0.905