Protein profile

KP13_00352

Isocitrate dehydrogenase kinase/phosphatase

Genome: KpKP13

Gene: AHE46909.1 aceK Structure source: AlphaFold + ColabFold UniProt A0A0H3GQ28
Export view
Amino acids 594
Annotations 12
Features 10
PDB binders 0
Druggability 0.95

Overview

Basic information about this protein and its source genome.

Accession
KP13_00352
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE46909.1 aceK
Status
annotated
Amino acids
594
Structure source
AlphaFold + ColabFold
EC
2.7.11.5 3.1.3.-
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0016791 Catalysis of the hydrolysis of a phosphoric monoester, releasing a phosphate. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0006006 The chemical reactions and pathways involving glucose, the aldohexose gluco-hexose. D-glucose is dextrorotatory and is sometimes known as dextrose; it is an important source of energy for living organisms and is found free as well as combined in homo- and hetero-oligosaccharides and polysaccharides. GO:0008772 Catalysis of the reaction: ATP + L-seryl-[isocitrate dehydrogenase] = ADP + H+ + O-phospho-L-seryl-[isocitrate dehydrogenase]. GO:0016208 Binding to AMP, adenosine monophosphate.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
92.5

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.95
Structure A0A0H3GQ28
Pocket Pocket 5
P2Rank 0.915
Structure A0A0H3GQ28
Pocket Pocket 1
ColabFold model
FPocket 0.969 · Pocket 7
P2Rank 0.935 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 111 / 4744 genomes with a hit
Normalized 0.023

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

2 EC 10 GO

Enzyme Commission (EC)

2

Gene Ontology (GO)

10
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0016791 Catalysis of the hydrolysis of a phosphoric monoester, releasing a phosphate.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0006006 The chemical reactions and pathways involving glucose, the aldohexose gluco-hexose. D-glucose is dextrorotatory and is sometimes known as dextrose; it is an important source of energy for living organisms and is found free as well as combined in homo- and hetero-oligosaccharides and polysaccharides.
  • GO:0008772 Catalysis of the reaction: ATP + L-seryl-[isocitrate dehydrogenase] = ADP + H+ + O-phospho-L-seryl-[isocitrate dehydrogenase].
  • GO:0016208 Binding to AMP, adenosine monophosphate.
  • GO:0004721 Catalysis of the reaction: a phosphoprotein + H2O = a protein + phosphate. Together with protein kinases, these enzymes control the state of phosphorylation of cellular proteins and thereby provide an important mechanism for regulating cellular activity.
  • GO:0004674 Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
  • GO:0006097 A modification of the TCA cycle occurring in some plants and microorganisms, in which isocitrate is cleaved to glyoxylate and succinate. Glyoxylate can then react with acetyl-CoA to form malate.
  • GO:0006099 A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle.

Sequence Features

Domain/signature hits from InterPro and related databases.

10 records
Show feature table
Start End DB Term Name
1 570 Hamap MF_00747 Isocitrate dehydrogenase kinase/phosphatase [aceK].
1 570 InterPro IPR010452 Isocitrate dehydrogenase kinasephosphatase
10 306 Pfam PF20423 Isocitrate dehydrogenase kinase/phosphatase (AceK), regulatory domain
10 306 InterPro IPR046854 Isocitrate dehydrogenase kinase/phosphatase (AceK), regulatory domain
1 572 PANTHER PTHR39559 -
1 572 InterPro IPR010452 Isocitrate dehydrogenase kinasephosphatase
1 575 PIRSF PIRSF000719 AceK
1 575 InterPro IPR010452 Isocitrate dehydrogenase kinasephosphatase
310 564 Pfam PF06315 Isocitrate dehydrogenase kinase/phosphatase (AceK) kinase domain
310 564 InterPro IPR046855 Isocitrate dehydrogenase kinase/phosphatase (AceK), kinase domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GQ28
AlphaFold — — full sequence — Viewing
ColabFold KP13_00352
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
5 0.95
20 0.788
1 0.549

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 23.8 0.892
2 8.67 0.464
3 7.87 0.418
4 4.75 0.21
5 2.54 0.071