Protein profile

KP13_01354

Transcription antitermination protein nusG

Genome: KpKP13

Gene: nusG AHE46944.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GPZ5
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Amino acids 181
Annotations 7
Features 35
PDB binders 0
Druggability 0.408

Overview

Basic information about this protein and its source genome.

Accession
KP13_01354
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
nusG AHE46944.1
Status
annotated
Amino acids
181
Structure source
AlphaFold + ColabFold
GO
GO:0140673 A chromatin remodeling process that reestablishes the chromatin structure following the passage of RNA polymerase II during transcription elongation, thus preventing cryptic transcription initiation. GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription. GO:0032784 Any process that modulates the frequency, rate or extent of transcription elongation, the extension of an RNA molecule after transcription initiation and promoter clearance by the addition of ribonucleotides catalyzed by a DNA-dependent RNA polymerase. GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0006354 The extension of an RNA molecule after transcription initiation and promoter clearance at a DNA-dependent RNA polymerase promoter by the addition of ribonucleotides catalyzed by an RNA polymerase. GO:0006353 The completion of transcription: the RNA polymerase pauses, the RNA-DNA hybrid dissociates, followed by the release of the RNA polymerase from its DNA template.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
100.0
DEG E-value
1.54e-132
Localization
Cytoplasmic
ColabFold pLDDT
77.77

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.408
Structure A0A0H3GPZ5
Pocket Pocket 6
P2Rank 0.087
Structure A0A0H3GPZ5
Pocket Pocket 1
ColabFold model
FPocket 0.397 · Pocket 9
P2Rank 0.188 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 291 / 4744 genomes with a hit
Normalized 0.061

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0140673 A chromatin remodeling process that reestablishes the chromatin structure following the passage of RNA polymerase II during transcription elongation, thus preventing cryptic transcription initiation.
  • GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
  • GO:0032784 Any process that modulates the frequency, rate or extent of transcription elongation, the extension of an RNA molecule after transcription initiation and promoter clearance by the addition of ribonucleotides catalyzed by a DNA-dependent RNA polymerase.
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0006354 The extension of an RNA molecule after transcription initiation and promoter clearance at a DNA-dependent RNA polymerase promoter by the addition of ribonucleotides catalyzed by an RNA polymerase.
  • GO:0006353 The completion of transcription: the RNA polymerase pauses, the RNA-DNA hybrid dissociates, followed by the release of the RNA polymerase from its DNA template.
  • GO:0031564 A positive regulation of gene expression mechanism that allows RNA polymerase to continue transcription beyond a termination site, thus allowing expression of downstream genes under specific conditions.

Sequence Features

Domain/signature hits from InterPro and related databases.

35 records
Show feature table
Start End DB Term Name
6 115 SMART SM00738 nusgn_4
6 115 InterPro IPR006645 NusG-like, N-terminal
1 117 FunFam G3DSA:3.30.70.940:FF:000001 Transcription termination/antitermination protein NusG
1 117 Gene3D G3DSA:3.30.70.940 -
1 117 InterPro IPR036735 NusG, N-terminal domain superfamily
61 73 PRINTS PR00338 Transcription termination factor NUSG signature
61 73 InterPro IPR001062 Transcription antitermination protein, NusG
139 154 PRINTS PR00338 Transcription termination factor NUSG signature
139 154 InterPro IPR001062 Transcription antitermination protein, NusG
157 173 PRINTS PR00338 Transcription termination factor NUSG signature
157 173 InterPro IPR001062 Transcription antitermination protein, NusG
5 180 PANTHER PTHR30265 RHO-INTERACTING TRANSCRIPTION TERMINATION FACTOR NUSG
5 180 InterPro IPR043425 NusG-like
130 162 Pfam PF00467 KOW motif
130 162 InterPro IPR005824 KOW
125 180 SUPERFAMILY SSF50104 Translation proteins SH3-like domain
125 180 InterPro IPR008991 Translation protein SH3-like domain superfamily
8 115 CDD cd09891 NGN_Bact_1
8 115 InterPro IPR047050 NusG, N-terminal
6 116 SUPERFAMILY SSF82679 N-utilization substance G protein NusG, N-terminal domain
6 116 InterPro IPR036735 NusG, N-terminal domain superfamily
6 181 Hamap MF_00948 Transcription termination/antitermination protein NusG [nusG].
6 181 InterPro IPR001062 Transcription antitermination protein, NusG
127 154 SMART SM00739 kow_9
127 154 InterPro IPR005824 KOW
9 180 NCBIfam TIGR00922 transcription termination/antitermination protein NusG
9 180 InterPro IPR001062 Transcription antitermination protein, NusG
123 181 Gene3D G3DSA:2.30.30.30 -
123 181 InterPro IPR014722 Ribosomal protein L2, domain 2
121 181 FunFam G3DSA:2.30.30.30:FF:000002 Transcription termination/antitermination factor NusG
125 180 CDD cd06091 KOW_NusG
164 173 ProSitePatterns PS01014 Transcription termination factor nusG signature.
164 173 InterPro IPR015869 Transcription antitermination protein, NusG, bacteria, conserved site
7 112 Pfam PF02357 Transcription termination factor nusG
7 112 InterPro IPR006645 NusG-like, N-terminal

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GPZ5
AlphaFold — — full sequence — Viewing
ColabFold KP13_01354
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
6 0.408
4 0.385

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 2.71 0.081