Protein profile

KP13_31625

Transcriptional activator rfaH

Genome: KpKP13

Gene: rfaH AHE46959.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GPY2
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Amino acids 162
Annotations 6
Features 15
PDB binders 0
Druggability 0.537

Overview

Basic information about this protein and its source genome.

Accession
KP13_31625
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
rfaH AHE46959.1
Status
annotated
Amino acids
162
Structure source
AlphaFold + ColabFold
GO
GO:0140673 A chromatin remodeling process that reestablishes the chromatin structure following the passage of RNA polymerase II during transcription elongation, thus preventing cryptic transcription initiation. GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription. GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). GO:0001073 Binds to DNA, typically within region of the promoter and transcribed region, to promote readthrough of a transcription termination site and thus extending the length of the RNA transcript produced. Examples of antitermination factors which bind DNA include the lambda Q protein. GO:0006354 The extension of an RNA molecule after transcription initiation and promoter clearance at a DNA-dependent RNA polymerase promoter by the addition of ribonucleotides catalyzed by an RNA polymerase.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
83.333
DEG E-value
5.15e-103
Localization
Unknown
ColabFold pLDDT
74.53

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.537
Structure A0A0H3GPY2
Pocket Pocket 13
P2Rank 0.019
Structure A0A0H3GPY2
Pocket Pocket 1
ColabFold model
FPocket 0.433 · Pocket 1
Core conservation Accessory gene
Roary core
CoreCruncher accessory
Gut microbiome 110 / 4744 genomes with a hit
Normalized 0.023

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0140673 A chromatin remodeling process that reestablishes the chromatin structure following the passage of RNA polymerase II during transcription elongation, thus preventing cryptic transcription initiation.
  • GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
  • GO:0001073 Binds to DNA, typically within region of the promoter and transcribed region, to promote readthrough of a transcription termination site and thus extending the length of the RNA transcript produced. Examples of antitermination factors which bind DNA include the lambda Q protein.
  • GO:0006354 The extension of an RNA molecule after transcription initiation and promoter clearance at a DNA-dependent RNA polymerase promoter by the addition of ribonucleotides catalyzed by an RNA polymerase.

Sequence Features

Domain/signature hits from InterPro and related databases.

15 records
Show feature table
Start End DB Term Name
3 96 CDD cd09892 NGN_SP_RfaH
1 162 Hamap MF_00951 Transcription antitermination protein RfaH [rfaH].
1 162 InterPro IPR010215 Transcription antitermination protein RfaH
1 162 Gene3D G3DSA:3.30.70.940 -
1 162 InterPro IPR036735 NusG, N-terminal domain superfamily
2 98 SUPERFAMILY SSF82679 N-utilization substance G protein NusG, N-terminal domain
2 98 InterPro IPR036735 NusG, N-terminal domain superfamily
1 100 SMART SM00738 nusgn_4
1 100 InterPro IPR006645 NusG-like, N-terminal
3 161 PANTHER PTHR30265 RHO-INTERACTING TRANSCRIPTION TERMINATION FACTOR NUSG
3 161 InterPro IPR043425 NusG-like
3 95 Pfam PF02357 Transcription termination factor nusG
3 95 InterPro IPR006645 NusG-like, N-terminal
4 159 NCBIfam TIGR01955 transcription/translation regulatory transformer protein RfaH
4 159 InterPro IPR010215 Transcription antitermination protein RfaH

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GPY2
AlphaFold — — full sequence — Viewing
ColabFold KP13_31625
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
13 0.537

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 1.46 0.02