KpKP13 Protein target profile

Protein hemY

Accession: KP13_01703

Gene: AHE46991.1 hemY 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GH46
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Length 398
Pocket druggability (P2Rank · AlphaFold DB model) 0.04
Functional annotation 0 EC 5 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Strong predicted binding pocket FPocket selected-pocket score 0.84. This is a computational pocket signal, not binding validation. Structure available AlphaFold DB model + ColabFold model

Risks to review

Microbiome similarity 109 of 4744 screened genomes (2.3%) have a significant similarity hit. This target also has a predicted binding pocket (FPocket 0.84) — check the ligand evidence before deprioritizing on off-target risk alone. Weak second pocket-predictor signal P2Rank predicted binding-site probability 0.04.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
2.3% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
89.15 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.04
Structure A0A0H3GH46
Pocket Pocket 1
Druggability (FPocket) 0.844
Structure A0A0H3GH46
Pocket Pocket 7
ColabFold model
P2Rank 0.059 · Pocket 1
FPocket 0.953 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 109 / 4744 genomes with a hit
Prevalence 2.3%

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GH46

Sequence

Primary amino-acid sequence viewer.

MLKILLLFALLIAGIVLGPMIAGHQGYVLIQTDNYNIETSVTGLVIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQVEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERATELAGDDLIPVEITRVRLQLARNENHAARHGIDKLLEITPRHPEVLRLAEQAYTRTGAWQSLLDIIPSMAKANVGDEAHRAALEQLAWVGLMDKALADGGSEGLREWWRNQSRKTRALVPLQVAMAERLIESDDHDTAQQIIIDGLKKQYDDRLVMPIPRLKTNNPEQLEKVLRQQIKAVGDRPLLWSTLGQSLMRHGEWQEASIAFRAALKQRPDAFDYAWLADALDRLHQPEEAAAMRRDGLLLTLQNNPQP

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

5
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0005515 Binding to a protein.
  • GO:0042168 The chemical reactions and pathways involving heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0006779 The chemical reactions and pathways resulting in the formation of any member of a large group of derivatives or analogs of porphyrin. Porphyrin consists of a ring of four pyrrole nuclei linked each to the next at their alpha positions through a methine group.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

24 records
Show feature table
Start End DB Term Name
5 27 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 3 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
1 18 SignalP_EUK SignalP-TM SignalP-TM
328 361 ProSiteProfiles PS50005 TPR repeat profile.
328 361 InterPro IPR019734 Tetratricopeptide repeat
1 26 Phobius SIGNAL_PEPTIDE Signal peptide region
71 296 Gene3D G3DSA:1.25.40.10 Tetratricopeptide repeat domain
71 296 InterPro IPR011990 Tetratricopeptide-like helical domain superfamily
304 396 Gene3D G3DSA:1.25.40.10 Tetratricopeptide repeat domain
304 396 InterPro IPR011990 Tetratricopeptide-like helical domain superfamily
26 132 Pfam PF07219 HemY protein N-terminus
26 132 InterPro IPR010817 HemY, N-terminal
97 382 SUPERFAMILY SSF48452 TPR-like
97 382 InterPro IPR011990 Tetratricopeptide-like helical domain superfamily
27 41 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
40 62 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
18 26 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
4 17 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
329 360 Pfam PF07719 Tetratricopeptide repeat
329 360 InterPro IPR013105 Tetratricopeptide repeat 2
42 62 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
63 398 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
4 386 NCBIfam TIGR00540 heme biosynthesis-associated TPR protein
4 386 InterPro IPR005254 Heme biosynthesis-associated TPR protein

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.04
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Surrounding area
Pocket 2 P2Rank #2
0.023
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Surrounding area
Pocket 3 P2Rank #3
0.017
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #7
0.844
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GH46
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_01703
ColabFold — — full sequence — Loaded