Protein profile

KP13_00585

Sensor protein cpxA

Genome: KpKP13

Gene: cpxA AHE47060.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GPN8
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Amino acids 457
Annotations 9
Features 50
PDB binders 3
Druggability 0.839

Overview

Basic information about this protein and its source genome.

Accession
KP13_00585
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
cpxA AHE47060.1
Status
annotated
Amino acids
457
Structure source
AlphaFold + ColabFold
EC
2.7.13.3
GO
GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor). GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
85.05

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.839
Structure A0A0H3GPN8
Pocket Pocket 1
P2Rank 0.838
Structure A0A0H3GPN8
Pocket Pocket 1
ColabFold model
FPocket 0.506 · Pocket 25
P2Rank 0.861 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 113 / 4744 genomes with a hit
Normalized 0.024

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0007155 The attachment of a cell, either to another cell or to an underlying substrate such as the extracellular matrix, via cell adhesion molecules.

Sequence Features

Domain/signature hits from InterPro and related databases.

50 records
Show feature table
Start End DB Term Name
181 234 Pfam PF00672 HAMP domain
181 234 InterPro IPR003660 HAMP domain
188 232 CDD cd06225 HAMP
5 27 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 455 PANTHER PTHR45528 SENSOR HISTIDINE KINASE CPXA
165 187 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
224 300 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
224 300 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
6 27 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
82 145 Pfam PF16527 Two-component sensor protein CpxA, periplasmic domain
82 145 InterPro IPR032404 Two-component sensor protein CpxA, periplasmic domain
184 237 SMART SM00304 HAMP_11
184 237 InterPro IPR003660 HAMP domain
238 299 Pfam PF00512 His Kinase A (phospho-acceptor) domain
238 299 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
185 457 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
245 455 ProSiteProfiles PS50109 Histidine kinase domain profile.
245 455 InterPro IPR005467 Histidine kinase domain
1 32 SignalP_EUK SignalP-TM SignalP-TM
27 147 Gene3D G3DSA:3.30.450.210 -
27 147 InterPro IPR038515 Two-component sensor protein CpxA, periplasmic domain superfamily
184 237 ProSiteProfiles PS50885 HAMP domain profile.
188 302 FunFam G3DSA:1.10.287.130:FF:000007 Sensor histidine kinase CpxA
236 296 CDD cd00082 HisKA
236 296 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
346 454 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
346 454 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
188 302 Gene3D G3DSA:1.10.287.130 -
238 300 SMART SM00388 HisKA_10
238 300 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
303 456 Gene3D G3DSA:3.30.565.10 -
303 456 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
345 455 SMART SM00387 HKATPase_4
345 455 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
303 456 FunFam G3DSA:3.30.565.10:FF:000011 Sensor histidine kinase CpxA
165 184 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
27 147 FunFam G3DSA:3.30.450.210:FF:000001 Sensor histidine kinase CpxA
350 453 CDD cd16949 HATPase_CpxA-like
289 453 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
289 453 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
28 164 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
439 452 PRINTS PR00344 Bacterial sensor protein C-terminal signature
439 452 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
415 433 PRINTS PR00344 Bacterial sensor protein C-terminal signature
415 433 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
398 408 PRINTS PR00344 Bacterial sensor protein C-terminal signature
398 408 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
380 394 PRINTS PR00344 Bacterial sensor protein C-terminal signature
380 394 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GPN8
AlphaFold — — full sequence — Viewing
ColabFold KP13_00585
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.839
23 0.823
8 0.478

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 11.94 0.632
2 1.57 0.024
3 1.23 0.011

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

53 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
4BIW
P0AE82 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO
RDC
3CGY
P0DM80 364.8 Da LogP 2.69 TPSA 96.4 ✓ Ro5 ✓ Clean C[C@@H]1C[C@@H]2[C@H](O2)\C=C/C=C/C(=O)Cc3c(c(c…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.