Protein profile

KP13_00590

HTH-type transcriptional activator rhaS

Genome: KpKP13

Gene: AHE47065.1 rhaS Structure source: AlphaFold + ColabFold UniProt A0A0H3GPN3
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Amino acids 290
Annotations 6
Features 29
PDB binders 0
Druggability 0.546

Overview

Basic information about this protein and its source genome.

Accession
KP13_00590
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE47065.1 rhaS
Status
annotated
Amino acids
290
Structure source
AlphaFold + ColabFold
GO
GO:0043565 Binding to DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding. GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription. GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons. GO:0019299 OBSOLETE. The chemical reactions and pathways involving rhamnose, the hexose 6-deoxy-L-mannose. Rhamnose occurs commonly as a compound of plant glycosides, in polysaccharides of gums and mucilages, and in bacterial polysaccharides. It is also a component of some plant cell wall polysaccharides and frequently acts as the sugar components of flavonoids. GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0045893 Any process that activates or increases the frequency, rate or extent of cellular DNA-templated transcription.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
89.47

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.546
Structure A0A0H3GPN3
Pocket Pocket 2
P2Rank 0.5
Structure A0A0H3GPN3
Pocket Pocket 1
ColabFold model
FPocket 0.547 · Pocket 7
P2Rank 0.54 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 76 / 4744 genomes with a hit
Normalized 0.016

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0043565 Binding to DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding.
  • GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
  • GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons.
  • GO:0019299 OBSOLETE. The chemical reactions and pathways involving rhamnose, the hexose 6-deoxy-L-mannose. Rhamnose occurs commonly as a compound of plant glycosides, in polysaccharides of gums and mucilages, and in bacterial polysaccharides. It is also a component of some plant cell wall polysaccharides and frequently acts as the sugar components of flavonoids.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0045893 Any process that activates or increases the frequency, rate or extent of cellular DNA-templated transcription.

Sequence Features

Domain/signature hits from InterPro and related databases.

29 records
Show feature table
Start End DB Term Name
13 285 Hamap MF_01534 HTH-type transcriptional activator RhaS [rhaS].
13 285 InterPro IPR023609 Transcription regulator HTH, RhaS
36 285 PANTHER PTHR46796 HTH-TYPE TRANSCRIPTIONAL ACTIVATOR RHAS-RELATED
28 166 Pfam PF02311 AraC-like ligand binding domain
28 166 InterPro IPR003313 AraC-type arabinose-binding/dimerisation domain
180 240 Gene3D G3DSA:1.10.10.60 -
251 266 PRINTS PR00032 AraC bacterial regulatory protein HTH signature
251 266 InterPro IPR020449 Transcription regulator HTH, AraC- type
266 282 PRINTS PR00032 AraC bacterial regulatory protein HTH signature
266 282 InterPro IPR020449 Transcription regulator HTH, AraC- type
186 284 ProSiteProfiles PS01124 Bacterial regulatory proteins, araC family DNA-binding domain profile.
186 284 InterPro IPR018060 DNA binding HTH domain, AraC-type
28 172 CDD cd06977 cupin_RhaR_RhaS-like_N
28 172 InterPro IPR047220 HTH-type transcriptional activator RhaR/RhaS, N-terminal domain
13 103 Gene3D G3DSA:2.60.120.10 Jelly Rolls
13 103 InterPro IPR014710 RmlC-like jelly roll fold
241 288 Gene3D G3DSA:1.10.10.60 -
205 283 Pfam PF12833 Helix-turn-helix domain
205 283 InterPro IPR018060 DNA binding HTH domain, AraC-type
235 284 SUPERFAMILY SSF46689 Homeodomain-like
235 284 InterPro IPR009057 Homeobox-like domain superfamily
182 233 SUPERFAMILY SSF46689 Homeodomain-like
182 233 InterPro IPR009057 Homeobox-like domain superfamily
199 282 SMART SM00342 aracneu4
199 282 InterPro IPR018060 DNA binding HTH domain, AraC-type
24 169 SUPERFAMILY SSF51215 Regulatory protein AraC
24 169 InterPro IPR037923 Transcription regulator HTH-like
236 278 ProSitePatterns PS00041 Bacterial regulatory proteins, araC family signature.
236 278 InterPro IPR018062 HTH domain AraC-type, conserved site

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GPN3
AlphaFold — — full sequence — Viewing
ColabFold KP13_00590
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.546
1 0.037
20 0.005
9 0.0

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 10.84 0.5
2 8.13 0.366
3 5.78 0.233
4 4.62 0.165
5 1.86 0.03