Protein target profile

KP13_00010

Low affinity potassium transport system protein kup

Genome: KpKP13 Gene: AHE47121.1 kup 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GPH2
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Length 622
Pocket druggability 0.644
Functional annotation 0 EC 3 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Moderate predicted binding pocket FPocket selected-pocket score 0.64. This supports review but is not experimental validation. Second pocket predictor agrees P2Rank predicted binding-site probability 0.70. Review the complete evidence below

Risks to review

Microbiome similarity 114 of 4744 screened genomes (2.4%) have a significant similarity hit. This target also has a predicted binding pocket (FPocket 0.64) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
2.4% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
48.763 Higher values support similarity to known essential genes.
DEG E-value
0.0 Smaller values mean stronger essential-gene similarity.

Localization

Localization
CytoplasmicMembrane

Structure confidence

ColabFold pLDDT
88.32 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

The selected pocket score is the FPocket value used for ranking after applying the curated structure priority. It estimates small-molecule pocket quality; it is not experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

FPocket 0.644
Structure A0A0H3GPH2
Pocket Pocket 36
P2Rank 0.701
Structure A0A0H3GPH2
Pocket Pocket 1
ColabFold model
FPocket 0.691 · Pocket 2
P2Rank 0.712 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 114 / 4744 genomes with a hit
Prevalence 2.4%

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GPH2

Sequence

Primary amino-acid sequence viewer.

MSTDNKQSLPALTLAAIGVVYGDIGTSPLYTLRECLSGQFGFGVERDAVFGFLSLIFWLLIFTVSIKYITFVMRADNAGEGGILTLMSLAGRNTSARMTSVLVILGLIGGSFFYGEVVITPAISVMSAIEGLEIIAPQLDTWIVPISIIVLTLLFVIQKHGTGMVGKLFAPIMLIWFLLLAVLGARSIYANPEVLQALNPYWAVHFFLQYKTVSFIALGAVVLSITGVEALYADMGHFGKLPIRVAWFSVVLPSLVLNYFGQGALLLKHPEAIKNPFFLLAPEWALIPMLIIATLATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMESGQIYIPFINWLLYVSVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSATVARKNWHWNKLFVGLMLVAFLCIDIPLFSANLDKIVSGGWLPLSLGMVMFTVMTTWKSERFRLLRRMHEHGNSLEAMISSLEKSPPVRVPGTAVYMSRALNVIPFALLHNLKHNKVLHERVILLTLRTEDAPYVHNVRRVQIEQLSPSFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMMETSFFMSHESLIIGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Gene Ontology (GO)

3
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0071805 A process in which a potassium ion is transported from one side of a membrane to the other.
  • GO:0015079 Enables the transfer of potassium ions (K+) from one side of a membrane to the other.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

47 records
Show feature table
Start End DB Term Name
245 267 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
335 357 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
115 133 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
157 167 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
363 383 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
210 232 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
317 336 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
209 233 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
9 17 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
1 8 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
245 267 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
287 316 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
7 29 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
384 394 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
419 440 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
23 47 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
168 189 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
94 114 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
337 357 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
74 93 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
14 544 Pfam PF02705 K+ potassium transporter
14 544 InterPro IPR003855 Potassium transporter
234 244 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
287 309 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
134 156 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
441 622 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
363 385 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
268 286 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
414 418 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
49 71 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
419 441 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
48 73 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
392 414 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
395 413 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
102 124 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
18 22 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
168 190 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
134 156 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
97 551 NCBIfam TIGR00794 potassium uptake protein
97 551 InterPro IPR003855 Potassium transporter
190 208 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
5 622 Hamap MF_01522 Low affinity potassium transport system protein Kup [kup].
5 622 InterPro IPR023051 Low affinity potassium transport system protein Kup
358 362 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 22 Phobius SIGNAL_PEPTIDE Signal peptide region
9 96 PANTHER PTHR30540 OSMOTIC STRESS POTASSIUM TRANSPORTER
9 96 InterPro IPR003855 Potassium transporter

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · FPocket

Druggability: high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Site 1 FPocket #36
0.644
Likely same site as P2Rank 2 3.7 Å 9 shared residues 90% of smaller site
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Surrounding area
Site 2 FPocket #19
0.479
Likely same site as P2Rank 3 1.3 Å 10 shared residues 100% of smaller site
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Surrounding area

Binding pockets · P2Rank

Probability: high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Site 1 P2Rank #1
0.701
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Surrounding area
Site 2 P2Rank #2
0.694
Likely same site as FPocket 36 3.7 Å 9 shared residues 90% of smaller site
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Surrounding area
Site 3 P2Rank #3
0.555
Likely same site as FPocket 19 1.3 Å 10 shared residues 100% of smaller site
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Surrounding area
Site 4 P2Rank #4
0.366
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Surrounding area
Site 5 P2Rank #5
0.357
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GPH2
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_00010
ColabFold — — full sequence — Loaded