Protein profile

KP13_32089

modification methylase HhaI

Genome: KpKP13

Gene: hhaIM AHE41892.1 Structure source: AlphaFold + ColabFold UniProt H6VZK2
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Amino acids 475
Annotations 5
Features 7
PDB binders 0
Druggability 0.298

Overview

Basic information about this protein and its source genome.

Accession
KP13_32089
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
hhaIM AHE41892.1
Status
annotated
Amino acids
475
Structure source
AlphaFold + ColabFold
EC
2.1.1.37
GO
GO:0008168 Catalysis of the transfer of a methyl group to an acceptor molecule. GO:0003886 Catalysis of the reaction: a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + H+ + S-adenosyl-L-homocysteine. GO:0009307 A defense process found in many bacteria and archaea that protects the organism from invading foreign DNA by cleaving it with a restriction endonuclease. The organism's own DNA is protected by methylation of a specific nucleotide, which occurs immediately following replication, in the same target site as the restriction enzyme. GO:0032259 The process in which a methyl group is covalently attached to a molecule.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
90.77

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.298
Structure H6VZK2
Pocket Pocket 26
P2Rank 0.797
Structure H6VZK2
Pocket Pocket 1
ColabFold model
FPocket 0.543 · Pocket 10
P2Rank 0.751 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 3 / 4744 genomes with a hit
Normalized 0.001

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0008168 Catalysis of the transfer of a methyl group to an acceptor molecule.
  • GO:0003886 Catalysis of the reaction: a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + H+ + S-adenosyl-L-homocysteine.
  • GO:0009307 A defense process found in many bacteria and archaea that protects the organism from invading foreign DNA by cleaving it with a restriction endonuclease. The organism's own DNA is protected by methylation of a specific nucleotide, which occurs immediately following replication, in the same target site as the restriction enzyme.
  • GO:0032259 The process in which a methyl group is covalently attached to a molecule.

Sequence Features

Domain/signature hits from InterPro and related databases.

7 records
Show feature table
Start End DB Term Name
228 320 Pfam PF00145 C-5 cytosine-specific DNA methylase
228 320 InterPro IPR001525 C-5 cytosine methyltransferase
257 321 PANTHER PTHR46098 TRNA (CYTOSINE(38)-C(5))-METHYLTRANSFERASE
141 364 Gene3D G3DSA:3.40.50.150 Vaccinia Virus protein VP39
141 364 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
188 451 SUPERFAMILY SSF53335 S-adenosyl-L-methionine-dependent methyltransferases
188 451 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_H6VZK2
AlphaFold — — full sequence — Viewing
ColabFold KP13_32089
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
26 0.298

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 6.95 0.359
2 3.1 0.104