Protein profile

KP13_01408

Type IV restriction endonuclease family protein

Genome: KpKP13

Gene: AHE47422.1 Structure source: AlphaFold + ColabFold UniProt A0A0U3AUH3
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Amino acids 195
Annotations 5
Features 11
PDB binders 0
Druggability 0.1

Overview

Basic information about this protein and its source genome.

Accession
KP13_01408
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE47422.1
Status
annotated
Amino acids
195
Structure source
AlphaFold + ColabFold
GO
GO:0003676 Binding to a nucleic acid. GO:0009307 A defense process found in many bacteria and archaea that protects the organism from invading foreign DNA by cleaving it with a restriction endonuclease. The organism's own DNA is protected by methylation of a specific nucleotide, which occurs immediately following replication, in the same target site as the restriction enzyme. GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). GO:0004519 Catalysis of the cleavage of ester linkages within nucleic acids by creating internal breaks. GO:0015666 Catalysis of endonucleolytic cleavage of DNA in a site-specific manner, resulting in double-strand breaks.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
91.34

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.1
Structure A0A0U3AUH3
Pocket Pocket 4
P2Rank 0.111
Structure A0A0U3AUH3
Pocket Pocket 1
ColabFold model
FPocket 0.534 · Pocket 10
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 6 / 4744 genomes with a hit
Normalized 0.001

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0003676 Binding to a nucleic acid.
  • GO:0009307 A defense process found in many bacteria and archaea that protects the organism from invading foreign DNA by cleaving it with a restriction endonuclease. The organism's own DNA is protected by methylation of a specific nucleotide, which occurs immediately following replication, in the same target site as the restriction enzyme.
  • GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
  • GO:0004519 Catalysis of the cleavage of ester linkages within nucleic acids by creating internal breaks.
  • GO:0015666 Catalysis of endonucleolytic cleavage of DNA in a site-specific manner, resulting in double-strand breaks.

Sequence Features

Domain/signature hits from InterPro and related databases.

11 records
Show feature table
Start End DB Term Name
1 5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
5 24 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
33 173 Gene3D G3DSA:3.40.1350.10 -
33 173 InterPro IPR011856 tRNA endonuclease-like domain superfamily
63 171 SUPERFAMILY SSF52980 Restriction endonuclease-like
63 171 InterPro IPR011335 Restriction endonuclease type II-like
26 195 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
6 25 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
16 171 PANTHER PTHR30015 MRR RESTRICTION SYSTEM PROTEIN
59 169 Pfam PF04471 Restriction endonuclease
59 169 InterPro IPR007560 Restriction endonuclease type IV, Mrr

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0U3AUH3
AlphaFold — — full sequence — Viewing
ColabFold KP13_01408
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 1.78 0.033