Protein profile

KP13_31572

Aminoglycoside N(6')-acetyltransferase

Genome: KpKP13

Gene: AHE47443.1 aacA4 Structure source: AlphaFold + ColabFold UniProt B6UYQ3
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Amino acids 201
Annotations 4
Features 11
PDB binders 4
Druggability 0.042

Overview

Basic information about this protein and its source genome.

Accession
KP13_31572
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE47443.1 aacA4
Status
annotated
Amino acids
201
Structure source
AlphaFold + ColabFold
EC
2.3.1.82
GO
GO:0016747 Catalysis of the transfer of an acyl group, other than amino-acyl, from one compound (donor) to another (acceptor). GO:0047663 Catalysis of the reaction: acetyl-CoA + kanamycin B = N(6')-acetylkanamycin B + CoA + H+. This is acetylation of the 6'-amino group of the 6-deoxy-6-aminoglucose ring. GO:0046677 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
90.11

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.042
Structure B6UYQ3
Pocket Pocket 1
P2Rank 0.961
Structure B6UYQ3
Pocket Pocket 1
ColabFold model
FPocket 0.032 · Pocket 1
P2Rank 0.957 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 8 / 4744 genomes with a hit
Normalized 0.002

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 3 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

3
  • GO:0016747 Catalysis of the transfer of an acyl group, other than amino-acyl, from one compound (donor) to another (acceptor).
  • GO:0047663 Catalysis of the reaction: acetyl-CoA + kanamycin B = N(6')-acetylkanamycin B + CoA + H+. This is acetylation of the 6'-amino group of the 6-deoxy-6-aminoglucose ring.
  • GO:0046677 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.

Sequence Features

Domain/signature hits from InterPro and related databases.

11 records
Show feature table
Start End DB Term Name
18 201 FunFam G3DSA:3.40.630.30:FF:000025 Aminoglycoside acetyltransferase
17 192 SUPERFAMILY SSF55729 Acyl-CoA N-acyltransferases (Nat)
17 192 InterPro IPR016181 Acyl-CoA N-acyltransferase
25 192 ProSiteProfiles PS51186 Gcn5-related N-acetyltransferase (GNAT) domain profile.
25 192 InterPro IPR000182 GNAT domain
14 201 Gene3D G3DSA:3.40.630.30 -
32 178 Pfam PF13523 Acetyltransferase (GNAT) domain
19 201 NCBIfam TIGR04431 AacA4 family aminoglycoside N(6')-acetyltransferase
19 201 InterPro IPR030971 Aminoglycoside N(6')-acetyltransferase, AacA4 type
80 144 CDD cd04301 NAT_SF
22 195 PANTHER PTHR31438 LYSINE N-ACYLTRANSFERASE C17G9.06C-RELATED

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_B6UYQ3
AlphaFold — — full sequence — Viewing
ColabFold KP13_31572
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 17.98 0.812
2 13.74 0.697
3 1.29 0.013

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

54 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
30N
4QC6
Q7ATH7 799.5 Da LogP -2.37 TPSA 383.9 3 viol. ✓ Clean CC(C)(COP(=O)(O)OP(=O)(O)OC[C@@H]1[C@H]([C@H]([…
9CS
2QIR
Q8GLI5 483.5 Da LogP -7.33 TPSA 288.4 2 viol. ✓ Clean C1[C@@H]([C@H]([C@@H]([C@H]([C@@H]1N)O[C@@H]2[C…
CHX
3F5B
Q5ZWV6 84.2 Da LogP 2.34 TPSA 0.0 ✓ Ro5 ✓ Clean C1CCCCC1
KAN
4QC6
Q7ATH7 484.5 Da LogP -7.29 TPSA 282.6 2 viol. ✓ Clean C1[C@H]([C@@H]([C@H]([C@@H]([C@H]1N)O[C@@H]2[C@…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.