Protein profile

KP13_02850

PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit dhaM

Genome: KpKP13

Gene: AHE42581.1 dhaM Structure source: AlphaFold + ColabFold UniProt A0A0H3GXV1
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Amino acids 473
Annotations 7
Features 43
PDB binders 2
Druggability 0.049

Overview

Basic information about this protein and its source genome.

Accession
KP13_02850
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE42581.1 dhaM
Status
annotated
Amino acids
473
Structure source
AlphaFold + ColabFold
EC
2.7.1.121
GO
GO:0047324 Catalysis of the reaction: glycerone + phosphoenolpyruvate = glycerone phosphate + pyruvate. GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor). GO:0019563 The chemical reactions and pathways resulting in the breakdown of glycerol, 1,2,3-propanetriol, a sweet, hygroscopic, viscous liquid, widely distributed in nature as a constituent of many lipids. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
87.56

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.049
Structure A0A0H3GXV1
Pocket Pocket 41
P2Rank 0.702
Structure A0A0H3GXV1
Pocket Pocket 1
ColabFold model
FPocket 0.654 · Pocket 1
P2Rank 0.191 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 37 / 4744 genomes with a hit
Normalized 0.008

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0047324 Catalysis of the reaction: glycerone + phosphoenolpyruvate = glycerone phosphate + pyruvate.
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
  • GO:0019563 The chemical reactions and pathways resulting in the breakdown of glycerol, 1,2,3-propanetriol, a sweet, hygroscopic, viscous liquid, widely distributed in nature as a constituent of many lipids.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.

Sequence Features

Domain/signature hits from InterPro and related databases.

43 records
Show feature table
Start End DB Term Name
276 390 Gene3D G3DSA:1.10.274.10 -
276 390 InterPro IPR036618 PtsI, HPr-binding domain superfamily
397 465 Pfam PF00391 PEP-utilising enzyme, mobile domain
397 465 InterPro IPR008279 PEP-utilising enzyme, mobile domain
168 175 ProSitePatterns PS00369 PTS HPR domain histidine phosphorylation site signature.
168 175 InterPro IPR001020 Phosphotransferase system, HPr histidine phosphorylation site
1 132 Gene3D G3DSA:3.40.50.510 -
1 132 InterPro IPR036662 Phosphotransferase system, mannose-type IIA component superfamily
273 387 SUPERFAMILY SSF47831 Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain
273 387 InterPro IPR036618 PtsI, HPr-binding domain superfamily
1 128 SUPERFAMILY SSF53062 PTS system fructose IIA component-like
1 128 InterPro IPR036662 Phosphotransferase system, mannose-type IIA component superfamily
2 127 NCBIfam TIGR02364 dihydroxyacetone kinase phosphoryl donor domain
2 127 InterPro IPR012844 Dihydroxyacetone kinase phosphotransferase subunit, N-terminal domain
391 471 Gene3D G3DSA:3.50.30.10 Phosphohistidine domain
370 471 SUPERFAMILY SSF52009 Phosphohistidine domain
370 471 InterPro IPR036637 Phosphohistidine domain superfamily
1 135 ProSiteProfiles PS51096 PTS_EIIA type-4 domain profile.
1 135 InterPro IPR004701 Phosphotransferase system, mannose-type IIA component
4 120 Pfam PF03610 PTS system fructose IIA component
4 120 InterPro IPR004701 Phosphotransferase system, mannose-type IIA component
1 134 PANTHER PTHR38594 PEP-DEPENDENT DIHYDROXYACETONE KINASE, PHOSPHORYL DONOR SUBUNIT DHAM
1 134 InterPro IPR039643 Dihydroxyacetone kinase phosphotransferase subunit DhaM
159 242 SUPERFAMILY SSF55594 HPr-like
159 242 InterPro IPR035895 HPr-like superfamily
161 223 CDD cd00367 PTS-HPr_like
161 223 InterPro IPR000032 Phosphocarrier protein HPr-like
157 243 Gene3D G3DSA:3.30.1340.10 -
157 243 InterPro IPR035895 HPr-like superfamily
156 243 ProSiteProfiles PS51350 PTS HPR domain profile.
156 243 InterPro IPR000032 Phosphocarrier protein HPr-like
168 184 PRINTS PR00107 Phosphocarrier protein signature
168 184 InterPro IPR000032 Phosphocarrier protein HPr-like
208 225 PRINTS PR00107 Phosphocarrier protein signature
208 225 InterPro IPR000032 Phosphocarrier protein HPr-like
193 208 PRINTS PR00107 Phosphocarrier protein signature
193 208 InterPro IPR000032 Phosphocarrier protein HPr-like
159 236 Pfam PF00381 PTS HPr component phosphorylation site
159 236 InterPro IPR000032 Phosphocarrier protein HPr-like
158 235 NCBIfam TIGR01003 HPr family phosphocarrier protein
158 235 InterPro IPR000032 Phosphocarrier protein HPr-like
272 370 Pfam PF05524 PEP-utilising enzyme, N-terminal
272 370 InterPro IPR008731 Phosphotransferase system, enzyme I N-terminal

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GXV1
AlphaFold — — full sequence — Viewing
ColabFold KP13_02850
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 10.81 0.58
2 1.14 0.009

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

2 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
OXL
2HWG
P08839 88.0 Da LogP -3.51 TPSA 80.3 ✓ Ro5 ✓ Clean C(=O)(C(=O)[O-])[O-]
PO3
2MP0
P08839 79.0 Da LogP -1.64 TPSA 63.2 ✓ Ro5 ✓ Clean [O-][P-](=O)[O-]

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.