Protein target profile
PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit dhaM
Accession: KP13_02850
Promising target candidate with multiple supporting evidence streams.
Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.
Main supporting evidence
Risks to review
Terms and data sources used on this page
PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.
AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.
ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.
pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.
FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.
Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.
PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.
ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.
ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.
LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.
Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.
DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.
Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.
EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.
KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.
Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.
Prioritization evidence
Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.
Off-target risk
- Human off-target
- No hit
- Gut microbiome similarity
- 0.8% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.
Essentiality
- Essential (DEG)
- N
- DEG identity (%)
- 0.0 Higher values support similarity to known essential genes.
Structure confidence
- ColabFold pLDDT
- 87.56 0-100 confidence; >70 supports local structural interpretation.
Binding-site evidence
AlphaFold DB / UniProt modelP2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.
Cross-references
External database identifiers for this protein, its structures, ligands, and metabolic reactions.
Sequence
Sequence
Primary amino-acid sequence viewer.
MVNLVIVSHSARLGEGVGELARQMLINDGCKLAIAAGIDDPDSPIGTDPLKVMEAIESVADTDHVLVMMDIGSALLSAETALDLLDPAIAAKVRLCAAPLVEGTLAATVSAASGAGIDKVIADAMSALEAKRVQLGLPSPTSDAAPAPMLADDGDTKSVSVIINNHNGLHVRPASKLVAALAGFNADLLLEKNGKCVKPDSLNQIALLQVRRNDKLRLLARGPDADAALAAFQALAADNFGESPAAKPAAAPATPERVEGAALRYPLALIQPLRPAAADAAREQQRLRQAIDQTLADLIALTELAENKFHADIAAIFAGHHTLLDDDDLFDAANDRLLTEQCTAEWAWHQVLMELSQQYRQLDDPYLQARYIDIEDILQRTLRHLQGVQERVPTPGEPTIIIADNIYPSTVLQLDASFVKGLCLRDGSEQAHGAIIARAAGIAWLSQQGEALNSVQPGETIVLDMRHQRLIRD
Functional annotations
Enzyme classification and Gene Ontology terms linked to this protein.
Subcellular localization
- Localization
- Cytoplasmic
Enzyme Commission (EC)
1Gene Ontology (GO)
6- GO:0047324 Catalysis of the reaction: glycerone + phosphoenolpyruvate = glycerone phosphate + pyruvate.
- GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
- GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
- GO:0019563 The chemical reactions and pathways resulting in the breakdown of glycerol, 1,2,3-propanetriol, a sweet, hygroscopic, viscous liquid, widely distributed in nature as a constituent of many lipids.
- GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
- GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
Sequence domains and features
Domain and signature matches imported from InterPro and related databases.
Show feature table
| Start | End | DB | Term | Name |
|---|---|---|---|---|
| 276 | 390 | Gene3D | G3DSA:1.10.274.10 | - |
| 276 | 390 | InterPro | IPR036618 | PtsI, HPr-binding domain superfamily |
| 397 | 465 | Pfam | PF00391 | PEP-utilising enzyme, mobile domain |
| 397 | 465 | InterPro | IPR008279 | PEP-utilising enzyme, mobile domain |
| 168 | 175 | ProSitePatterns | PS00369 | PTS HPR domain histidine phosphorylation site signature. |
| 168 | 175 | InterPro | IPR001020 | Phosphotransferase system, HPr histidine phosphorylation site |
| 1 | 132 | Gene3D | G3DSA:3.40.50.510 | - |
| 1 | 132 | InterPro | IPR036662 | Phosphotransferase system, mannose-type IIA component superfamily |
| 273 | 387 | SUPERFAMILY | SSF47831 | Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain |
| 273 | 387 | InterPro | IPR036618 | PtsI, HPr-binding domain superfamily |
| 1 | 128 | SUPERFAMILY | SSF53062 | PTS system fructose IIA component-like |
| 1 | 128 | InterPro | IPR036662 | Phosphotransferase system, mannose-type IIA component superfamily |
| 2 | 127 | NCBIfam | TIGR02364 | dihydroxyacetone kinase phosphoryl donor domain |
| 2 | 127 | InterPro | IPR012844 | Dihydroxyacetone kinase phosphotransferase subunit, N-terminal domain |
| 391 | 471 | Gene3D | G3DSA:3.50.30.10 | Phosphohistidine domain |
| 370 | 471 | SUPERFAMILY | SSF52009 | Phosphohistidine domain |
| 370 | 471 | InterPro | IPR036637 | Phosphohistidine domain superfamily |
| 1 | 135 | ProSiteProfiles | PS51096 | PTS_EIIA type-4 domain profile. |
| 1 | 135 | InterPro | IPR004701 | Phosphotransferase system, mannose-type IIA component |
| 4 | 120 | Pfam | PF03610 | PTS system fructose IIA component |
| 4 | 120 | InterPro | IPR004701 | Phosphotransferase system, mannose-type IIA component |
| 1 | 134 | PANTHER | PTHR38594 | PEP-DEPENDENT DIHYDROXYACETONE KINASE, PHOSPHORYL DONOR SUBUNIT DHAM |
| 1 | 134 | InterPro | IPR039643 | Dihydroxyacetone kinase phosphotransferase subunit DhaM |
| 159 | 242 | SUPERFAMILY | SSF55594 | HPr-like |
| 159 | 242 | InterPro | IPR035895 | HPr-like superfamily |
| 161 | 223 | CDD | cd00367 | PTS-HPr_like |
| 161 | 223 | InterPro | IPR000032 | Phosphocarrier protein HPr-like |
| 157 | 243 | Gene3D | G3DSA:3.30.1340.10 | - |
| 157 | 243 | InterPro | IPR035895 | HPr-like superfamily |
| 156 | 243 | ProSiteProfiles | PS51350 | PTS HPR domain profile. |
| 156 | 243 | InterPro | IPR000032 | Phosphocarrier protein HPr-like |
| 168 | 184 | PRINTS | PR00107 | Phosphocarrier protein signature |
| 168 | 184 | InterPro | IPR000032 | Phosphocarrier protein HPr-like |
| 208 | 225 | PRINTS | PR00107 | Phosphocarrier protein signature |
| 208 | 225 | InterPro | IPR000032 | Phosphocarrier protein HPr-like |
| 193 | 208 | PRINTS | PR00107 | Phosphocarrier protein signature |
| 193 | 208 | InterPro | IPR000032 | Phosphocarrier protein HPr-like |
| 159 | 236 | Pfam | PF00381 | PTS HPr component phosphorylation site |
| 159 | 236 | InterPro | IPR000032 | Phosphocarrier protein HPr-like |
| 158 | 235 | NCBIfam | TIGR01003 | HPr family phosphocarrier protein |
| 158 | 235 | InterPro | IPR000032 | Phosphocarrier protein HPr-like |
| 272 | 370 | Pfam | PF05524 | PEP-utilising enzyme, N-terminal |
| 272 | 370 | InterPro | IPR008731 | Phosphotransferase system, enzyme I N-terminal |
3D structure
Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.
How colors and pocket overlays are used
Pocket details Inspect a specific pocket, or open the full viewer
- Method
- -
- Score
- -
- Visible layer
- -
- Residues
- -
- Pocket properties
- -
Selecting a pocket opens its details and centers the viewer without clearing other active layers. Use Focus this pocket when you want to hide the rest; use Surface for the wider residue environment.
Binding pockets · P2Rank
Probability: high ≥ 0.5 · medium 0.2–0.49 · low < 0.2
Binding pockets · P2Rank
Probability: high ≥ 0.5 · medium 0.2–0.49 · low < 0.2
Binding pockets · FPocket
Druggability: high ≥ 0.7 · medium 0.4–0.69 · low < 0.4
All structural evidence
Structural evidence
0 + 2Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.
| Entry | Method | Resolution | Chain | Coverage | Links | Status |
|---|---|---|---|---|---|---|
|
AlphaFold DB
AF_A0A0H3GXV1
|
AlphaFold DB | — | — | full sequence | — | Viewing |
|
ColabFold
KP13_02850
|
ColabFold | — | — | full sequence | — | Loaded |
Ligand evidence
Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.
Structural ligand evidence is available for this target.
Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in Target, use Open crystal to inspect it in the structure viewer.
No PDB structure with a co-crystallized ligand found for this exact protein.
Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.
Experimental bioactivity from ChEMBL measured directly on this protein. Score = pchembl (−log Ki/IC₅₀; higher = more potent).
No ChEMBL bioactivity data found for this exact protein.
Bioactivity inferred from similar proteins in ChEMBL. Score = pchembl (−log Ki/IC₅₀; higher = more potent).
No ChEMBL hits found through similar proteins.
Proposed virtual-screening candidates from ZINC. Score = Tanimoto similarity to a known binder (0–1; higher = more similar).
No virtual-screening candidates for this protein.
PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.