Genome KpKP13

Protein target profile

hypothetical protein

Accession: KP13_01040

Gene: AHE43432.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GT73
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Length 183
Pocket druggability (P2Rank · AlphaFold DB model) 0.021
Functional annotation 1 EC 4 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Structure available AlphaFold DB model + ColabFold model

Risks to review

Microbiome similarity 144 of 4744 screened genomes (3.0%) have a significant similarity hit. Mixed conservation Core/accessory calls are not fully concordant. Weak predicted binding pocket FPocket selected-pocket score 0.21. Weak second pocket-predictor signal P2Rank predicted binding-site probability 0.02.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
3.0% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
53.086 Higher values support similarity to known essential genes.
DEG E-value
1.5999999999999999e-55 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
89.87 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.021
Structure A0A0H3GT73
Pocket Pocket 1
Druggability (FPocket) 0.211
Structure A0A0H3GT73
Pocket Pocket 5
ColabFold model
P2Rank 0.061 · Pocket 1
FPocket 0.202 · Pocket 16
Core conservation Accessory gene
Roary core
CoreCruncher accessory
Gut microbiome 144 / 4744 genomes with a hit
Prevalence 3.0%

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GT73

Sequence

Primary amino-acid sequence viewer.

MKQKTSLSEEDQVLFRQLMTGTRKIKQDTIVHRPQRKKITEVAPKRLLQEQVDNSHYFSDEFQPLLNTEGSTKYVRPDVSHFELKKLRRGDYSPELFLDLHGLTQQQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHIMAFHQAPKEYGGDAALLILIEVEEWQPPELP

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Subcellular localization

Localization
Cytoplasmic

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0016787 Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc.
  • GO:0004521 Catalysis of the cleavage of ester linkages within ribonucleic acid by creating internal breaks.
  • GO:0019843 Binding to a ribosomal RNA.
  • GO:0072344 A process of cytosolic translational elongation that takes place when a cytosolic ribosome has stalled during translation, and results in freeing the ribosome from the stalled translation complex.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

13 records
Show feature table
Start End DB Term Name
1 174 Hamap MF_01042 UPF0115 protein YfcN [yfcN].
1 174 InterPro IPR022990 Uncharacterised protein family UPF0115
49 176 Gene3D G3DSA:3.30.1370.110 -
49 176 InterPro IPR036063 Smr domain superfamily
98 172 Pfam PF01713 Smr domain
98 172 InterPro IPR002625 Smr domain
95 173 SMART SM00463 SMR_2
95 173 InterPro IPR002625 Smr domain
96 150 SUPERFAMILY SSF160443 SMR domain-like
96 150 InterPro IPR036063 Smr domain superfamily
1 178 PANTHER PTHR35562 DNA ENDONUCLEASE SMRA-RELATED
98 173 ProSiteProfiles PS50828 Smr domain profile.
98 173 InterPro IPR002625 Smr domain

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.021
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Surrounding area
Pocket 2 P2Rank #2
0.006
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #5
0.211
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GT73
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_01040
ColabFold — — full sequence — Loaded