Protein profile

KP13_01848

Type 4 prepilin-like proteins leader peptide-processing enzyme bifunctional protein

Genome: KpKP13

Gene: pulO AHE46288.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GRY2
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Amino acids 266
Annotations 8
Features 36
PDB binders 0
Druggability 0.133

Overview

Basic information about this protein and its source genome.

Accession
KP13_01848
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
pulO AHE46288.1
Status
annotated
Amino acids
266
Structure source
AlphaFold + ColabFold
EC
2.1.1.- 3.4.23.43
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0004190 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which a water molecule bound by the side chains of aspartic residues at the active center acts as a nucleophile. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0008168 Catalysis of the transfer of a methyl group to an acceptor molecule. GO:0032259 The process in which a methyl group is covalently attached to a molecule. GO:0006465 OBSOLETE. The proteolytic removal of a signal peptide from a protein during or after transport to a specific location in the cell.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
49.434
DEG E-value
1.02e-70
Localization
CytoplasmicMembrane
ColabFold pLDDT
93.09

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.133
Structure A0A0H3GRY2
Pocket Pocket 15
P2Rank 0.697
Structure A0A0H3GRY2
Pocket Pocket 1
ColabFold model
FPocket 0.818 · Pocket 1
P2Rank 0.709 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 12 / 4744 genomes with a hit
Normalized 0.003

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

2 EC 6 GO

Enzyme Commission (EC)

2

Gene Ontology (GO)

6
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0004190 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which a water molecule bound by the side chains of aspartic residues at the active center acts as a nucleophile.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0008168 Catalysis of the transfer of a methyl group to an acceptor molecule.
  • GO:0032259 The process in which a methyl group is covalently attached to a molecule.
  • GO:0006465 OBSOLETE. The proteolytic removal of a signal peptide from a protein during or after transport to a specific location in the cell.

Sequence Features

Domain/signature hits from InterPro and related databases.

36 records
Show feature table
Start End DB Term Name
245 262 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
132 149 PRINTS PR00864 Type IV prepilin cysteine protease (C20) family signature
132 149 InterPro IPR014032 Peptidase A24A, prepilin type IV, bacterial
200 211 PRINTS PR00864 Type IV prepilin cysteine protease (C20) family signature
200 211 InterPro IPR014032 Peptidase A24A, prepilin type IV, bacterial
189 199 PRINTS PR00864 Type IV prepilin cysteine protease (C20) family signature
189 199 InterPro IPR014032 Peptidase A24A, prepilin type IV, bacterial
212 227 PRINTS PR00864 Type IV prepilin cysteine protease (C20) family signature
212 227 InterPro IPR014032 Peptidase A24A, prepilin type IV, bacterial
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
123 231 Pfam PF01478 Type IV leader peptidase family
123 231 InterPro IPR000045 Prepilin type IV endopeptidase, peptidase domain
136 146 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
203 233 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
192 202 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
234 244 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
148 165 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
114 118 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
167 171 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
172 191 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
13 33 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
263 266 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
115 248 Gene3D G3DSA:1.20.120.1220 -
147 166 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
34 91 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
169 191 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 12 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
13 265 PANTHER PTHR30487 TYPE 4 PREPILIN-LIKE PROTEINS LEADER PEPTIDE-PROCESSING ENZYME
240 262 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
91 113 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
21 109 Pfam PF06750 Bacterial Peptidase A24 N-terminal domain
21 109 InterPro IPR010627 Peptidase A24A, N-terminal
92 113 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
203 225 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
117 135 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
119 135 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GRY2
AlphaFold — — full sequence — Viewing
ColabFold KP13_01848
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 10.23 0.55
2 4.21 0.171
3 4.12 0.166
4 1.94 0.04
5 1.84 0.036